Recombinant Rat IL-4 Protein

Name: Recombinant Rat IL-4 Protein
SKU: rp186535
Availability: InStock

货号: rp186535

COA

有货

级别和纯度: 无载体 ? 生物活性 ? ActiBioPure™ ? 高性能 ? His标签 ? ≥95%(SDS-PAGE) expressed in E. coli; 见COA

Accession #

P20096

蛋白标签

His

表达系统

E. coli

内毒素水平

<1.0 EU/μg

生物活性

Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 2.98 ng/mL.

规格

库存

价格

数量

10μg

rp186535-10μg

期货

¥599.90

50μg

rp186535-50μg

期货

¥1,699.90

100μg

rp186535-100μg

期货

¥2,699.90

1mg

rp186535-1mg

期货

¥15,999.90

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为什么选择此级别

ActiBioPure™,His标签,无载体,生物活性,高性能级提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存,避免反复冻融。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。

规格

产品名称

Recombinant Rat IL-4 Protein

别名

重组大鼠IL-4蛋白 | 重组大鼠白细胞介素-4蛋白

英文别名

规格或纯度

无载体, 生物活性, ActiBioPure™, 高性能, His标签, ≥95%(SDS-PAGE), expressed in E. coli; 见COA

生化机理

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Rat IL-4 is synthesized with a 24 aa signal sequence. Mature rat IL-4 shares 41%, 43%, and 59% aa sequence identity with bovine, human, and mouse IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL‑13 mediated signals. IL-4 is primarily expressed by Th2‑biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG1 and IgE in rodent B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.

生物活性

Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 2.98 ng/mL.

表达系统

E. coli

种属

大鼠(Rat)

氨基酸

25-147 aa

序列

MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDEIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFLDANLAGSGSGHMHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTWELQCNDSPLREIINTLNQVTEKGTPCTEMFVPDVLTATRNTTENELICRASRVLRKFYFPRDVPPCLKNKSGVLGELRKLCRGVSGLNSLRSCTVNESTLTTLKDFLESLKSILRGKYLQSCTSMS

蛋白标签

His

无载体

Yes

无动物源

来源

重组表达

预测分子量

30.7 kDa

SDS-PAGE

27.6 kDa, under reducing conditions; 27.0 kDa, under non-reducing conditions.

分子类型

蛋白质

产品线

生物产品

储存与运输

物理形态

冻干(Lyophilized)

储存缓冲液

Lyophilized from PBS, pH 7.4.

浓度

expressed in E. coli; 见COA

复溶

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

储存条件

-20°C储存,避免反复冻融

运输条件

超低温运输

稳定性与储存

-20℃保存 6个月；复溶后建议分装，避免反复冻融。

图片

Protein Bioactivity: Recombinant Rat IL-4 Protein (rp186535)

Recombinant Rat IL-4 Protein (rp186535) - Protein Bioactivity

Measured in a cell proliferation assay using mouse splenocytes. The ED50 for this effect is typically 2.98 ng/mL.

SDS-PAGE: Recombinant Rat IL-4 Protein (rp186535)

Recombinant Rat IL-4 Protein (rp186535) - SDS-PAGE

3 μg/lane of Recombinant Rat IL-4 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 27.6 & 57.8 kDa under reducing conditions and 27.0 & 54.4 kDa under non-reducing conditions. Multiple disulfide bonds in the protein sequence result in the protein existing as a polymer under non-reducing conditions (Uniprot ID: P20096).

技术文档

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该级别的完整质量属性和验收标准。

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高级数据

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

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技术文档和文章

TH细胞分化通路的激活基础、谱系决定与功能分化

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探索主题:

细胞因子免疫偏向 Treg TH细胞分化 CD4+T细胞 TH1 TH2 TH3样调节性细胞共刺激 TH17

Activation Basis, Lineage Commitment, and Functional Differentiation of TH Cell Differentiation Pathways

目录：技术文章

探索主题:

Cytokines immune polarization Treg TH1 TH2 TH17 TH cell differentiation CD4+ T cell TH3-like regulatory cells Costimulation

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Recombinant Rat IL-4 Protein

储存与运输

质量文档

文献证明

规格

技术文档

📋 安全数据表 (SDS)

✅ 分析证书 (COA)

📊 产品数据表

🔬 规格说明书

高级数据

摩尔浓度计算器

稀释计算器

复溶计算器

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