T-cell surface glycoprotein CD3 gamma chain, also known as CD3G, is a 20 kDa single-pass type I membrane protein that is part of the TCR-CD3 complex. CD3 gamma forms a heterodimer with CD3 epsilon which associates with TCR beta, while CD3δ/CD3 epsilon heterodimer associates with TCR alpha. CD3 gamma /CD3 epsilon /TCR beta joins CD3δ/CD3 epsilon /TCR alpha and recruits a CD3 zeta homodimer to form the complete TCR-CD3 complex. CD3 chains contain one cytoplasmic ITAM domain which mediates intracellular signal transduction. The extracellular domain of CD3 gamma contains one disulfide bond and 2 N-linked glycosylation sites. The ECD of human CD3 gamma shares 60% and 62% sequence identity with mouse and rat CD3 gamma, respectively. The biologic roles of each CD3 chain and their relative contribution to the signals transduced through the TCR/CD3 complex and to downstream activation events are still uncertain, whether they may be specialized or redundant. Deficiencies in CD3 gamma can cause impairments in immune function, particularly in Treg cells which may contribute to autoimmune disorders.
Post-translational: Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8 (PubMed:2470098).
Phosphorylated also by PKC; leading to the TCR complex down-regulation (PubMed:8187769).Phosphorylated on Tyr residues after T-cell receptor triggering by LCK in association with CD4/CD8.
生物活性
Immobilized Recombinant Human CD3 gamma Protein (rp188220) at 1.0 μg/mL can bind Recombinant CD3G Antibody (Ab094727) with the EC50 of 8.0 ng/mL.
41.5-48.3 kDa, under reducing conditions; 84.9-101.2 & 175.7-211.0 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human CD3 gamma Protein (rp188220) - ELISA Immobilized Recombinant Human CD3 gamma Protein (rp188220) at 1.0 μg/mL can bind Recombinant CD3G Antibody (Ab094727) with the EC50 of 8.0 ng/mL.
Recombinant Human CD3 gamma Protein (rp188220) - SDS-PAGE 3 μg/lane of Recombinant Human CD3 gamma Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 41.5-48.3 kDa under reducing conditions and 84.9-101.2 & 175.7-211.0 kDa under non-reducing conditions. Fc fusion proteins can be linked by disulfide bonds in the Fc hinge region to form stable dimers (PMID: 23665380).