无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE),See COA
生化机理
Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, is a potent activator of phosphofructokinase, which is a rate-limiting enzyme of glycolysis. Highly phosphorylated PFKFB3 protein was found in human tumor cells, vascular endothelial cells, and smooth muscle cells. Fructose 2,6-bisphosphate (Fru-2,6-BP) is an allosteric activator of 6-phosphofructo-1-kinase (PFK-1), a rate-limiting enzyme and essential control point in glycolysis. The concentration of PFK2 depends on the activity of the bifunctional enzyme, 6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (PFK-2 / FBPase). PFK2 controls the glycolytic flux via the allosteric activator fructose 2,6-bisphosphate. Because of its proto-oncogenic character, the PFK-2/FBPase-2 of the PFKFB3 gene is assumed to play a critical role in tumorigenesis. The hypoxia-inducible form of 6-phosphofructo-2-kinase / fructose-2,6-bisphosphatase (PFKFB3) plays a crucial role in the progression of cancerous cells by enabling their glycolytic pathways even under severely hypoxic conditions.
Post-translational: Phosphorylation by AMPK stimulates activity.
生物活性
Immobilized Recombinant Human PFKFB3 Protein (rp188263) at 1.0 μg/mL can bind Recombinant PFKFB3 Antibody (Ab121416) with the EC50 of 5.0 ng/mL.
Recombinant Human PFKFB3 Protein (rp188263) - ELISA
Immobilized Recombinant Human PFKFB3 Protein (rp188263) at 1.0 μg/mL can bind Recombinant PFKFB3 Antibody (Ab121416) with the EC₅₀ of 5.0 ng/mL.
Recombinant Human PFKFB3 Protein (rp188263) - SDS-PAGE
3 μg/lane of Recombinant Human PFKFB3 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 57.1 kDa under reducing conditions and 57.1 kDa under non-reducing conditions.