Immobilized Recombinant Human DNA Polymerase Beta Protein (rp188411) at 1.0 μg/mL can bind Recombinant DNA Polymerase beta Antibody (Ab100090) with the EC50 of 3.2 ng/mL.
DNA directed DNA polymerase beta | DNA pol beta | DNA polymerase beta | DNA polymerase beta subunit | DPOLB_HUMAN | MGC125976 | Pol B | Pol beta | POLB | Polymerase (DNA directed) beta
规格或纯度
无载体,生物活性,ActiBioPure™,His标签,≥95%(SDS-PAGE),见COA
生化机理
Repair polymerase that plays a key role in base-excision repair (PubMed:10556592, PubMed:9207062, PubMed:9572863). During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap (PubMed:10556592, PubMed:17526740, PubMed:9556598, PubMed:9572863, PubMed:9614142). Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase (PubMed:9614142).
Post-translational: Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation.
生物活性
Immobilized Recombinant Human DNA Polymerase Beta Protein (rp188411) at 1.0 μg/mL can bind Recombinant DNA Polymerase beta Antibody (Ab100090) with the EC50 of 3.2 ng/mL.
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human DNA Polymerase Beta Protein (rp188411) - ELISA Immobilized Recombinant Human DNA Polymerase Beta Protein (rp188411) at 1.0 μg/mL can bind Recombinant DNA Polymerase beta Antibody (Ab100090) with the EC₅₀ of 3.2 ng/mL.
Recombinant Human DNA Polymerase Beta Protein (rp188411) - SDS-PAGE 3 μg/lane of Recombinant Human DNA Polymerase Beta Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 40.1 kDa.
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