Immobilized Recombinant Human S100A9 Protein (rp209720) at 1.0 μg/mL can bind Recombinant Human S100A8 Protein (rp183594) with the EC50 of 189.6 ng/mL.
CAGBMigration inhibitory factor-related protein 14 | Calgranulin B | calgranulin-B | Calprotectin L1H subunit | CFAGMRP-14,60B8AG | CGLB | L1AG | LIAG | MAC387 | MIF | MRP-14 | MRP14Leukocyte L1 complex heavy chain | NIF | P14 | protein S100-A9 | S100 cal
规格或纯度
无载体, 生物活性, ActiBioPure™, ≥95%(SDS-PAGE)
生化机理
S100A9, also known as MRP14 and Calgranulin B, is an approximately 14 kDa pro-inflammatory protein in the S100 family of calcium-binding proteins. It is up-regulated in neutrophils and monocytes at sites of inflammation (e.g., psoriasis, rheumatoid arthritis, cardiac ischemia) and is present at elevated concentrations in rheumatoid arthritis synovial fluid. Human S100A9 contains two EF-hand calcium-binding motifs and shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively. S100A9 is a noncovalent homodimer that can also noncovalently heterodimerize with S100A8; in the presence of calcium and zinc, the homodimer and heterodimers will form tetramers. Human S100A9 can be phosphorylated at Thr113 near the C-terminus, a residue that is not conserved in the mouse protein. Human S100A9 can also be modified by S-glutathionylation and oxidation. The S100A8/A9 heterodimer binds to fatty acids such as arachidonic acid, while neither monomer does. S100A9 and the heterodimer promote neutrophil infiltration and degranulation at sites of inflammation and inflammatory cytokine production by monocytes. S100A9 also promotes cartilage matrix destruction in osteoarthritis as well as RAGE-dependent cardiac fibrosis and remodeling. In the brain, the S100A9 homodimer promotes neuronal cytotoxicity, although this is reduced by its binding to amyloid-beta peptide.
Post-translational: Phosphorylated. Phosphorylation inhibits activation of tubulin polymerization. S-nitrosylation of Cys-3 is implicated in LDL(ox)-induced S-nitrosylation of GAPDH at 'Cys-247' through a transnitrosylase mechanism involving a iNOS-S100A8/9 complex (PubMed:25417112). Methylation at His-105 by METTL9 reduces zinc-binding without affecting heterodimerization with S100A8.
生物活性
Immobilized Recombinant Human S100A9 Protein (rp209720) at 1.0 μg/mL can bind Recombinant Human S100A8 Protein (rp183594) with the EC50 of 189.6 ng/mL.
12.2 & 27.2 kDa, under reducing conditions; 12.2 & 27.2 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, 1mM TCEP, pH 7.4
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 1.0 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human S100A9 Protein (rp209720) - Binding Activity Immobilized Recombinant Human S100A9 Protein (rp209720) at 1.0 μg/mL can bind Recombinant Human S100A8 Protein (rp183594) with the EC50 of 189.6 ng/mL.
Recombinant Human S100A9 Protein (rp209720) - SDS-PAGE 3 μg/lane of Recombinant Human S100A9 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 12.2 & 27.2 kDa.
ZJ24F1214679
危险品化学品经营许可证（带存储）