Apolipoprotein A1 (ApoA1) is a 28 kDa glycoprotein that is the major protein component of high density lipoprotein (HDL) particles. HDL particles play a central role in the reverse transport of cholesterol from peripheral tissues to the liver. HDL is known as "good cholesterol" due to its ability to facilitate the removal of cholesterol from macrophage foam cells in atherosclerotic plaques and thereby retard the progression of disease. ApoA1 is secreted from hepatocytes with variable amounts of lipidation. Disc-like HDL particles increase in size and adopt a spherical shape as they are loaded with additional lipids and cholesterol by ABC family transporters. HDL particle interaction with lipid-supplying cells is mediated by ApoA1 binding to the scavenger receptor SR-A1. HDL particles are further modified by the circulating enzymes LCAT (which converts cholesterol to cholesteryl esters), CETP (which transfers cholesteryl esters to LDL/VLDL particles), and PLTP (which transfers phospholipids from HDL to LDL/VLDL particles). Upon HDL particle return to the liver, ApoA1 binds to the scavenger receptor SR-B1 and the beta chain of ATP synthase on hepatocytes. Hepatocytes internalize the particles and pass the cholesterol into bile for excretion. Polymorphisms of ApoA1 are associated with dysregulation of HDL levels and cholesterol homeostasis. ApoA1 is catabolized in the kidney following its binding to Cubulin on renal proximal tubule epithelial cells. ApoA1, either as a free molecule or in lipidated particulate form, induces the release of insulin from pancreatic islets in a process that is dependent on ABCA1 and SR-B1. Mature human ApoA1 shares 65% and 62% aa sequence identity with mouse and rat ApoA1, respectively.
Post-translational: Glycosylated.Palmitoylated.Phosphorylation sites are present in the extracellular medium.
23.6 & 25.2 & 25.8 kDa, under reducing conditions; 23.6 & 25.2 & 25.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 6个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human Apolipoprotein A-I Protein (rp214424) - SDS-PAGE 3 μg/lane of Recombinant Human Apolipoprotein A-I Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 23.6 & 25.2 & 25.8 kDa. ApoA-I is cleaved by specific proteases in E.coli, generating N-terminal or C-terminal truncated variants that form fragments of approximately 23 to 25 kDa (PMID: 16183915).
危险品化学品经营许可证（带存储）