Hormone involved in the regulation of erythrocyte proliferation and differentiation and the maintenance of a physiological level of circulating erythrocyte mass (PubMed:28283061). Binds to EPOR leading to EPOR dimerization and JAK2 activation thereby activating specific downstream effectors, including STAT1 and STAT3 (PubMed:9774108).
生物活性
Measured in a cell proliferation assay using TF1 human erythroleukemic cells. The ED50 for this effect is 0.18 ng/mL
28.3-40.1 kDa, under reducing conditions; 28.3-39.5 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月;复溶后建议分装,避免反复冻融。
图片
Recombinant Human Erythropoietin/EPO Protein (rp226954) - Protein Bioactivity Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED₅₀ for this effect is 0.18 ng/mL
Recombinant Human Erythropoietin/EPO Protein (rp226954) - SDS-PAGE 3 μg/lane of Recombinant Human Erythropoietin/EPO Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 28.3-40.1 kDa under reducing conditions and 28.3-39.5 kDa under non-reducing conditions.