Insulin-like growth factor 1 (IGF-1 or IGF-I), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-1 is synthesized as two precursor isoforms with N- and alternate C terminal propeptides. These isoforms are differentially expressed by various tissues. The 7.6 kDa mature IGF 1 is identical between isoforms and is generated by proteolytic removal of the N- and C terminal regions. Mature human IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-1, respectively, and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF2. Circulating IGF-1 is produced by hepatocytes, while local IGF-1 is produced by many other tissues in which it has paracrine effects. IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally. IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism. It plays an important role in muscle regeneration and tumor progression. IGF-1 binds IGF1R, IGF2R, and the insulin receptor, although its effects are mediated primarily by IGF1R. IGF-1 association with IGF binding proteins increases its plasma half life and modulates its interactions with receptors.
生物活性
Measured in a cell proliferation assay using MCF-7 human breast cancer cell line. The ED50 for this effect is typically 34.3 ng/mL.
7.6 kDa, under reducing conditions; 7.6 & 15.7, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
-20℃保存 12个月；复溶后建议分装，避免反复冻融。
图片
Recombinant Human IGF-I/IGF-1 Protein (rp228953) - Protein Bioactivity
Measured in a cell proliferation assay using MCF-7 human breast cancer cell line. The ED₅₀ for this effect is typically 9.08 ng/mL.
Recombinant Human IGF-I/IGF-1 Protein (rp228953) - SDS-PAGE
3 μg/lane of Recombinant Human IGF-I/IGF-1 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the bands at 7.6 kDa under reducing conditions and 7.6 & 15.7 kDa under non-reducing conditions.
IGF-I/IGF-1 can form a stable dimer on its own, which is mediated by a disulfide bond connecting the C-terminal helices of the α-subunits (referred to as α-CT for short) on the two monomers (PMID: 31594955).
ZJ25F1230265
危险品化学品经营许可证（带存储）