Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as a phosphorylated and variably glycosylated molecule. It exerts actions through its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined as an essential role in directing the transition from innate to acquired immunity.
Post-translational: N- and O-glycosylated.
生物活性
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.16 ng/mL.
19.6 kDa, under reducing conditions; 18.9 & 41.8 kDa, under non-reducing conditions.
分子类型
蛋白质
产品线
生物产品
储存与运输
物理形态
冻干(Lyophilized)
储存缓冲液
Lyophilized from PBS, pH 7.4.
浓度
见COA
复溶
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute at 0.5 mg/mL in sterile distilled water. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C for 1 year. Avoid freeze/thaw cycle.
图片
Recombinant Human IL-6 Protein (rp228960) - Protein Bioactivity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.16 ng/mL.
Recombinant Human IL-6 Protein (rp228960) - SDS-PAGE 3 μg/lane of Recombinant Human IL-6 Protein was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing a band at 19.6 kDa under reducing conditions and 18.9 & 41.8 kDa under non-reducing conditions. The observation of IL-6 Protein as both dimers and monomers under non-reducing (NR) conditions suggests that a portion of the protein forms dimers. This dimerization may be mediated by (or stabilized by) disulfide bonds (Uniprot: P05231).