苏丹黑B染色液

Name: 苏丹黑B染色液
SKU: S1508362
Availability: InStock

CAS: 4197-25-5 货号: S1508362 分子式: C₂₉H₂₄N₆ 分子量: 456.54 Beilstein号: 723248 EC号: 224-087-1 PubChem CID: 61336

20篇引用文献 COA

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级别和纯度: BioReagent ? 生物染色剂 ? 用于显微镜 ?

储存条件

避光,室温

运输条件

常规运输

应用

细胞染色

规格

库存

价格

数量

2×50ml

S1508362-2×50ml

期货

¥309.90

2×100ml

S1508362-2×100ml

现货

¥569.90

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概述规格名称和识别符技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 化学和物理性质安全和危险性（GHS）此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

BioReagent,生物染色剂,用于显微镜级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

避光,室温。常规运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 20 篇同行评审文献引用。

概述

实验室对于中性脂肪染色有多种方法，例如采用苏丹黑 B、苏丹Ⅱ、苏丹Ⅲ、苏丹Ⅳ、油红O法等。苏丹染料一般用于传统方法，现在偶氮染料油红O更适合用于脂肪的染色。油红O是一类很强的脂溶剂和染脂剂，它虽然与磷脂结合力稍差，但却较易和甘油三脂结合呈小脂滴状。苏丹黑B染色液的主要用途在于显示类脂质的异常沉着和组织器官的脂肪变性，以及肝、肾、心等实质脏器中经常发生的脂肪变性，细胞内出现多数中性脂肪滴；鉴别和诊断脂肪组织中所发生的肿瘤及其性质。实验过程中，标本不采用含有乙醇的固定液，如实际情况需要固定可以采用10%福尔马林，也不采用石蜡切片，需用碳蜡切片或冰冻切片。

S1508362	Component	2×50 mL	2×100 mL	Storage
S1508362A	Sudan Black B Stain	50 mL	100 mL	RT. Store in the dark
S1508362B	Nuclear Fast Red Stain	50 mL	100 mL	RT. Store in the dark

操作步骤（仅供参考）：

1、新鲜组织低温切片，一般在温度-20℃～-25℃范围。如果实验样本为脂肪瘤，应调节温度至-30℃。

2、冰冻切片 5～10μm(6～8μm为佳)，贴于载玻片上。

3、70%乙醇稍微浸洗一下。

4、放入Sudan black B stain浸染。

5、浓度为70%的乙醇洗去多余染液。

6、蒸馏水浸洗 1min。

7、加入 Nuclear fast red Stain 淡染细胞核。

8、(可选)镜下观察如染色过深，可用酸性分化液分化数秒。

9、自来水稍洗后，蒸馏水同样稍洗一下。

10、用滤纸吸去切片及周围的水分，让其稍微干燥。

11、甘油明胶或阿拉伯糖胶封固。

染色结果:

中性脂肪: 黑色

磷脂: 灰黑色

细胞核: 红色

注意事项：

1、标本不宜采用含有乙醇的固定液、也不宜用石蜡切片，需用冰冻切片。

2、在染色过程中为了防止染料发生沉淀。将切片入染液时应密封，请勿与流动空气相接触，避免溶液挥发时发生沉淀。

3、冰冻切片较易着色，Mayer苏木素复染时应避免过染。

4、苏丹染料容易褪色，应保存于密闭环境。

5、甘油明胶封固的样本，保存时间不长。如需长期保存，可以用中性树胶将盖玻片与载玻片交界的边缘封闭。

There are various methods for neutral fat staining in the laboratory, such as Sudan Black B, Sudan Ⅱ, Sudan Ⅲ, Sudan Ⅳ, and Oil Red O staining. Sudan dyes are generally used in traditional methods, while the azo dye Oil Red O is now more suitable for fat staining. Oil Red O is a highly effective lipid solvent and lipochrome; although its binding affinity to phospholipids is relatively weak, it readily binds to triglycerides to form small lipid droplets. Sudan Black B Stain is mainly used to demonstrate abnormal deposition of lipids, fatty degeneration of tissues and organs, as well as the fatty degeneration commonly occurring in parenchymal organs such as the liver, kidneys, and heart, where numerous neutral fat droplets appear within cells. It also aids in the identification and diagnosis of tumors arising in adipose tissue and their pathological properties. During the experiment, fixatives containing ethanol should not be used for specimens. If fixation is necessary, 10% formalin can be applied. In addition, paraffin sections are not recommended; instead, carbowax sections or frozen sections should be adopted.

S1508362	Component	2×50 mL	2×100 mL	Storage
S1508362A	Sudan Black B Stain	50 mL	100 mL	RT. Store in the dark
S1508362B	Nuclear Fast Red Stain	50 mL	100 mL	RT. Store in the dark

Operating Procedures (For Reference Only)

1. repare cryosections of fresh tissues at a temperature typically ranging from -20℃ to -25℃. For lipoma specimens, adjust the temperature to -30℃.

2. Cut frozen sections with a thickness of 5–10 μm (6–8 μm is optimal) and mount them on glass slides.

3. Rinse the sections briefly with 70% ethanol.

4. Immerse the sections in Sudan Black B Stain for staining.

5. Wash away excess stain with 70% ethanol.

6. Rinse the sections with distilled water for 1 minute.

7. Stain the cell nuclei lightly with Nuclear Fast Red Stain.

8. (Optional) If overstaining is observed under the microscope, differentiate the sections with acid differentiation solution for a few seconds.

9. Rinse briefly with tap water, followed by a quick rinse with distilled water.

10. Blot excess water from the sections and their surroundings with filter paper and allow them to air-dry slightly.

11. Mount the sections with glycerin jelly or gum arabic.

Staining Results

Neutral Fat: Black

Phospholipids Grayish: Black

Cell Nuclei: Red

Precautions

1. Do not use ethanol-containing fixatives or prepare paraffin sections for specimens; frozen sections are required.

2. To prevent dye precipitation during staining, seal the container when immersing sections in the stain. Avoid contact with flowing air to prevent precipitation caused by solvent evaporation.

3. Frozen sections stain easily. Avoid overstaining when counterstaining with Mayer's hematoxylin.

4. Sudan dyes are prone to fading and should be stored in a sealed environment.

5. Specimens mounted with glycerin jelly have a short shelf life. For long-term preservation, seal the edges between the coverslip and slide with neutral balsam.

规格

规格或纯度

BioReagent, 生物染色剂, 用于显微镜

稳定性与储存

各组分在相应的储存条件下保质期1年。

英文名称

Sudan Black B Staining Solution

储存条件

避光,室温

运输条件

常规运输

产品线

生物产品

名称和识别符

EC号

224-087-1

分子类型

生物试剂/缓冲液

Isomeric SMILES

CC1(NC2=C3C(=C(C=C2)N=NC4=CC=C(C5=CC=CC=C54)N=NC6=CC=CC=C6)C=CC=C3N1)C

PubChem CID

61336

分子量

456.54

Beilstein号

723248

Reaxy-Rn

8878215

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

下载 SDS →

✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

查询 COA →

📊 产品数据表

产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

查看规格说明 →

高级数据

化学和物理性质

敏感性

Light-sensitive

熔点

120-124°C

安全和危险性（GHS）

象形图

GHS08

信号词

Warning

危险声明

H341: 怀疑引起遗传缺陷

预防措施声明

P405: 密闭存放

P501: 将内容物/容器处理到。。。

P281: 根据需要使用个人防护设备。

P203: 使用前，获取、阅读并遵守所有安全说明。

P318: 如果暴露或担心，请就医。

WGK Germany

RTECS

SD4431500

个人防护装备

Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到2个结果

批号(Lot Number)	证书类型	货号
ZJ26F0737150	分析证书	S1508362
ZJ26F0332497	分析证书	S1508362

技术文档和文章

s1508362_苏丹黑b染色液产品说明书.pdf

血液细胞化学染色的原理、分类与临床实验应用

目录：技术文章

探索主题:

PAS染色血液细胞化学染色骨髓涂片外周血涂片过氧化物酶染色苏丹黑B染色非特异性酯酶染色特异性酯酶染色中性粒细胞碱性磷酸酶铁染色白血病鉴别

Principles, Classification, and Clinical Laboratory Applications of Hematologic Cytochemical Staining

目录：技术文章

探索主题:

PAS Staining Sudan Black B staining Hematologic Cytochemical Staining Bone Marrow Smear Peripheral Blood Smear Peroxidase Staining Nonspecific Esterase Staining Specific Esterase Staining Neutrophil Alkaline Phosphatase Iron Staining Leukemia Differentiation

常见脂质染色方法的原理、适用场景与结果判读

目录：技术文章

探索主题:

BODIPY 组织化学染色胆固醇油红O 尼罗红脂质染色苏丹黑B 苏丹Ⅲ 苏丹Ⅳ 菲利平锇酸中性脂滴髓鞘脂质沉积

Principles, Applicable Scenarios, and Result Interpretation of Common Lipid Staining Methods

目录：技术文章

探索主题:

BODIPY Cholesterol Oil Red O Nile Red lipid staining Sudan Black B Sudan III Sudan IV filipin osmium tetroxide neutral lipid droplets myelin lipid deposition histochemical staining

偶氮类染料型指示剂的结构特征、变色机制与应用选择

目录：技术文章

探索主题:

甲基红刚果红染料型指示剂偶氮染料甲基橙橙黄G 铬黑T PAN PAR 钙镁试剂络合滴定金属显色剂组织染色

Structural Characteristics, Color-Change Mechanisms, and Application Selection of Azo Dye-Type Indicators

目录：技术文章

探索主题:

Methyl Red Congo Red dye-type indicator PAN PAR azo dye methyl orange Orange G Eriochrome Black T calmagite complexometric titration metal color reagent tissue staining

尿沉渣显微镜检查的形态学基础、方法流程与结果判读

目录：技术文章

探索主题:

细菌白细胞尿沉渣显微镜检查红细胞上皮细胞管型尿结晶真菌寄生虫黏液丝相差显微镜尿液有形成分

Morphological Basis, Method Workflow, and Result Interpretation of Urinary Sediment Microscopy

目录：技术文章

探索主题:

Bacteria urinary sediment microscopy red blood cells white blood cells epithelial cells casts urinary crystals fungi parasites mucus threads phase-contrast microscopy formed elements in urine

TSA 酪胺信号放大实用指南：低丰度靶标检测信号弱时，如何选试剂、优化流程、降低背景？

目录：技术文章

探索主题:

免疫细胞化学 ICC 免疫组织化学 IHC HRP 辣根过氧化物酶原位杂交 ISH TSA 酪胺信号放大 FISH Immunocytochemistry 荧光原位杂交

Practical Guide to TSA Tyramide Signal Amplification: How to Select Reagents, Optimize the Workflow, and Reduce Background When Detection Signals Are Weak for Low-Abundance Targets

目录：技术文章

探索主题:

ICC IHC Immunohistochemistry Horseradish peroxidase In situ hybridization TSA tyramide signal amplification Fluorescence In Situ Hybridization

特殊染色方法选择与病理诊断应用：纤维、糖类、脂质、色素和病原体染色

目录：技术文章

探索主题:

Masson三色染色 PAS染色特殊染色网状纤维染色弹力纤维染色脂肪染色色素染色病原微生物染色

Selection of Special Staining Methods and Their Applications in Pathological Diagnosis: Fibers, Carbohydrates, Lipids, Pigments, and Pathogen Staining

目录：技术文章

探索主题:

PAS Staining Masson’s trichrome staining Special Staining lipid staining reticular fiber staining elastic fiber staining pigment staining pathogenic microorganism staining

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