苏丹黑B染色液

Name: 苏丹黑B染色液
SKU: S1508441
Availability: InStock

CAS: 4197-25-5 货号: S1508441 分子式: C₂₉H₂₄N₆ 分子量: 456.54 Beilstein号: 723248 EC号: 224-087-1 PubChem CID: 61336

20篇引用文献 COA

有货

级别和纯度: BioReagent ? 生物染色剂 ? 用于显微镜 ?

储存条件

避光,室温

运输条件

常规运输

应用

细胞染色

规格

库存

价格

数量

3×20ml

S1508441-3×20ml

期货

¥219.90

3×50ml

S1508441-3×50ml

现货

¥479.90

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概述规格名称和识别符技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 化学和物理性质安全和危险性（GHS）此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

BioReagent,生物染色剂,用于显微镜级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

避光,室温。常规运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 20 篇同行评审文献引用。

概述

脂质 (Lipid)是中性脂肪、类脂及其衍生物的总称，其共同的物理特性是不溶于水，易溶于有机溶剂 (如乙醇、乙醚等)。人体的脂肪主要有两种：1、储存脂肪，如中性脂肪，主要分布于皮下、肾、胰腺等部位。2、结构脂肪，如类脂 (磷脂、糖脂、胆固醇等)，主要分布于细胞内。中性脂肪 (Neutral fat)是由三分子脂肪酸和一分子甘油组成的脂类，呈中性。中性脂肪染色经常采用苏丹Ⅱ、苏丹Ⅲ、苏丹Ⅳ、苏丹黑B、油红O法等。传统方法采用苏丹染料，最近发现偶氮染料油红O更适合脂肪的染色。油红O是很强的脂溶剂和染脂剂，较易与甘油三脂结合呈小脂滴状，与磷脂结合力稍差。

苏丹黑B染色液 (细胞专用)简称SBB染色溶液，其原理是苏丹黑B属于脂溶性染料，可溶解于细胞质内的含脂结构中，使脂肪、磷脂、胆固醇等脂类现棕黑或深黑色，并定位于细胞质。神经磷脂、脑磷脂等行苏丹黑B染色后，呈阳性反应；肾透明细胞癌、卵巢纤维瘤等行苏丹黑染色后，呈阴性反应。

S1508441	Component	3×20 mL	3×50 mL	Storage
S1508441A	SBB Fixative	20 mL	50 mL	RT. Store in the dark
S1508441B	SBB Stain A	10 mL	25 mL	RT. Store in the dark
S1508441C	SBB Stain B	10 mL	25 mL	RT
S1508441D	Wright Stain	20 mL	50 mL	RT. Store in the dark

临用前，取B、C混合，即为SBB Stain，即配即用

自备材料：

1、载玻片

2、70%乙醇

3、蒸馏水

4、显微镜

操作步骤(仅供参考)：

1、制备新鲜骨髓、血液涂片，入 SBB Fixative 固定。

2、取出涂片，放于流通的空气中。

3、入新配制好的 SBB Stain，室温浸染。

4、入 Sudan black B stain，浸染。

5、乙醇洗去多余染液，蒸馏水浸洗。

6、 Wright Stain 染色。

染色结果：

中性脂肪：棕黑色或深黑色颗粒

细胞核：红色

粒细胞系除早期原粒细胞阴性外，分化好的原粒细胞以下阶段细胞随细胞成熟而阳性反应增强，衰老中性粒细胞反应程度减弱单核细胞系弱阳性，淋巴细胞系为阴性。浆细胞及巨核细胞均为阴性。嗜酸性粒细胞和 Auer 小体呈强阳性反应。

阴性对照：取相同样本入乙醚、氯仿、丙酮等量混合液，溶解一般脂肪，然后用预冷丙酮溶解磷脂，再进行 SBB 染色，结果呈阴性反应。

注意事项:

1、标本不宜采用含有乙醇的固定液。

2、苏丹染料容易褪色，应密闭保存。

3、应防止染料发生沉淀，可置于血平皿内，两端放置小竹签，血膜向下架空染色。

4、神经磷脂、脑磷脂SBB染色呈阳性，有利于对类脂质沉积病的诊断。

5、为了您的安全和健康，请穿实验服并戴一次性手套操作。

Lipids are a general term for neutral fats, lipids, and their derivatives. Their common physical characteristic is insolubility in water and solubility in organic solvents (e.g., ethanol, ether, etc.). There are two main types of fats in the human body: Storage fats: such as neutral fats, mainly distributed in subcutaneous tissues, kidneys, pancreas, and other parts. Structural fats: such as lipids (phospholipids, glycolipids, cholesterol, etc.), mainly distributed in cells. Neutral fats are lipids composed of three molecules of fatty acids and one molecule of glycerol, and they are neutral in nature. Staining methods for neutral fats often include Sudan Ⅱ, Sudan Ⅲ, Sudan Ⅳ, Sudan Black B, and Oil Red O staining. Traditional methods use Sudan dyes, but recent studies have found that the azo dye Oil Red O is more suitable for fat staining. Oil Red O is a strong lipid solvent and lipochrome; it binds easily to triglycerides to form small lipid droplets, but has a relatively weak binding affinity for phospholipids.

Sudan Black B Staining Solution (Cell-specific), abbreviated as SBB Stain, works on the principle that Sudan Black B is a liposoluble dye. It can dissolve in lipid-containing structures in the cytoplasm, staining fats, phospholipids, cholesterol, and other lipids brown-black or dark black and localizing them in the cytoplasm. Sphingomyelin, cephalin, and other substances show positive reactions after SBB staining; conversely, clear cell renal cell carcinoma, ovarian fibroma, and other tissues show negative reactions after SBB staining.

S1508441	Component	3×20 mL	3×50 mL	Storage Conditions
S1508441A	SBB Fixative	20 mL	50 mL	RT. Store in the dark
S1508441B	SBB Stain A	10 mL	25 mL	RT. Store in the dark
S1508441C	SBB Stain B	10 mL	25 mL	RT
S1508441D	Wright Stain	20 mL	50 mL	RT. Store in the dark

Preparation before use: Mix Reagent B and Reagent C to prepare SBB Stain. The stain should be used immediately after preparation.

Materials to be prepared by the user:

1. Glass slides

2. 70% ethanol

3. Distilled water

4. Microscope

Operating Procedures (For Reference Only)

1. Prepare smears of fresh bone marrow or blood, and fix them with SBB Fixative.

2. Take out the smears and air-dry them in circulating air.

3. Immerse the smears in freshly prepared SBB Stain and incubate at room temperature.

4. Transfer the smears to Sudan Black B Stain for further staining.

5. Wash away excess stain with ethanol, followed by rinsing with distilled water.

6. Perform counterstaining with Wright Stain.

Staining Results

Neutral Fats: Brown-black or dark black granules

Cell Nuclei: Red

In the granulocyte series, except for early myeloblasts (negative), cells from the well-differentiated myeloblast stage onwards show progressively stronger positive reactions with cell maturation. Senescent neutrophils show weakened positive reactions. Monocytes show weak positive reactions. Lymphocytes show negative reactions. Plasma cells and megakaryocytes are both negative. Eosinophils and Auer bodies show strong positive reactions.

Negative Control: Treat the same sample with a mixture of equal volumes of ether, chloroform, and acetone to dissolve general fats; then use pre-cooled acetone to dissolve phospholipids. Subsequent SBB staining of the treated sample should yield negative results.

Precautions

1. Do not use ethanol-containing fixatives for specimen preservation.

2. Sudan dyes are prone to fading and should be stored in sealed containers.

3. To prevent dye precipitation, place the smears in a petri dish with small bamboo sticks at both ends, and stain the smears with the blood film facing downward (suspended above the stain solution).

4. Sphingomyelin and cephalin yield positive results with SBB staining, which is helpful for the diagnosis of lipid storage diseases.

5. For your safety and health, wear a lab coat and disposable gloves during operation.

规格

规格或纯度

BioReagent, 生物染色剂, 用于显微镜

稳定性与储存

各组分在相应的储存条件下保质期1年。

英文名称

Sudan Black B Staining Solution

储存条件

避光,室温

运输条件

常规运输

产品线

生物产品

名称和识别符

EC号

224-087-1

分子类型

生物试剂/缓冲液

Isomeric SMILES

CC1(NC2=C3C(=C(C=C2)N=NC4=CC=C(C5=CC=CC=C54)N=NC6=CC=CC=C6)C=CC=C3N1)C

PubChem CID

61336

分子量

456.54

Beilstein号

723248

Reaxy-Rn

8878215

技术文档

📋 安全数据表 (SDS)

全面的危险、操作、储存及法规合规文件。

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✅ 分析证书 (COA)

批次质量数据。输入批号以获取对应 COA。

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产品规格和应用的快速参考摘要。

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🔬 规格说明书

该级别的完整质量属性和验收标准。

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高级数据

化学和物理性质

敏感性

Light-sensitive

熔点

120-124°C

安全和危险性（GHS）

象形图

GHS08

信号词

Warning

危险声明

H341: 怀疑引起遗传缺陷

预防措施声明

P405: 密闭存放

P501: 将内容物/容器处理到。。。

P281: 根据需要使用个人防护设备。

P203: 使用前，获取、阅读并遵守所有安全说明。

P318: 如果暴露或担心，请就医。

WGK Germany

RTECS

SD4431500

个人防护装备

Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter

质检证书（CoA,COO,BSE/TSE 和分析图谱)

C of A & Other Certificates(BSE/TSE, COO):

输入批号以搜索分析图谱:

通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！

找到1个结果

批号(Lot Number)	证书类型	货号
ZJ26F0332458	分析证书	S1508441

技术文档和文章

s1508441_苏丹黑b染色液产品说明书.pdf

血液细胞化学染色的原理、分类与临床实验应用

目录：技术文章

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PAS染色血液细胞化学染色骨髓涂片外周血涂片过氧化物酶染色苏丹黑B染色非特异性酯酶染色特异性酯酶染色中性粒细胞碱性磷酸酶铁染色白血病鉴别

Principles, Classification, and Clinical Laboratory Applications of Hematologic Cytochemical Staining

目录：技术文章

探索主题:

PAS Staining Sudan Black B staining Hematologic Cytochemical Staining Bone Marrow Smear Peripheral Blood Smear Peroxidase Staining Nonspecific Esterase Staining Specific Esterase Staining Neutrophil Alkaline Phosphatase Iron Staining Leukemia Differentiation

常见脂质染色方法的原理、适用场景与结果判读

目录：技术文章

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BODIPY 组织化学染色胆固醇油红O 尼罗红脂质染色苏丹黑B 苏丹Ⅲ 苏丹Ⅳ 菲利平锇酸中性脂滴髓鞘脂质沉积

Principles, Applicable Scenarios, and Result Interpretation of Common Lipid Staining Methods

目录：技术文章

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BODIPY Cholesterol Oil Red O Nile Red lipid staining Sudan Black B Sudan III Sudan IV filipin osmium tetroxide neutral lipid droplets myelin lipid deposition histochemical staining

偶氮类染料型指示剂的结构特征、变色机制与应用选择

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甲基红刚果红染料型指示剂偶氮染料甲基橙橙黄G 铬黑T PAN PAR 钙镁试剂络合滴定金属显色剂组织染色

Structural Characteristics, Color-Change Mechanisms, and Application Selection of Azo Dye-Type Indicators

目录：技术文章

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Methyl Red Congo Red dye-type indicator PAN PAR azo dye methyl orange Orange G Eriochrome Black T calmagite complexometric titration metal color reagent tissue staining

尿沉渣显微镜检查的形态学基础、方法流程与结果判读

目录：技术文章

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细菌白细胞尿沉渣显微镜检查红细胞上皮细胞管型尿结晶真菌寄生虫黏液丝相差显微镜尿液有形成分

Morphological Basis, Method Workflow, and Result Interpretation of Urinary Sediment Microscopy

目录：技术文章

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Bacteria urinary sediment microscopy red blood cells white blood cells epithelial cells casts urinary crystals fungi parasites mucus threads phase-contrast microscopy formed elements in urine

TSA 酪胺信号放大实用指南：低丰度靶标检测信号弱时，如何选试剂、优化流程、降低背景？

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