计算溶液所需的质量、体积或浓度。
精子细胞BWW培养基 (无菌)
有货
为什么选择此级别
BioReagent,无菌,用于细胞培养 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
正常精液是一种混合物，在射精时由睾丸和附睾的分泌物及悬浮其中的精子与前列腺、精囊腺和尿道球腺的分泌物混合而成，最终射出的混合物是一种粘稠的液体，精子分析的方法有很多，其中可通过培养进行检测。
精子细胞BWW培养基又称为获能培养液 (capacitating medium)，主要由系列盐离子、酚红、葡萄糖、丙酮酸钠、BSA等以及抗生素组成，每1000ml BWW培养基中含100,000U青霉素钠盐和100mg链霉素，是一种旨在用于广谱动物和人体精子细胞获能处理的常用营养液，其标准化成分配方依据Biggers-Whitten-Whittingham (BWW)研究小组在1971年发表的成果，适合于各种动物和人体精子细胞处理，该试剂经无菌处理。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
无菌离心管、离心机、细胞培养箱
操作步骤 (仅供参考)：
1、取干净的精液样本室温放置30-60min，使之充分液化。
2、制备精子细胞 (仅供参考，不是必须步骤)：
(1) 上泳法：取一个无菌的15ml锥底离心管，加入1ml液化的精液，在其上方轻轻加入Earle培养液1.2ml，45°倾斜试管，37℃孵育1h，轻轻竖立试管，取出最上层的1ml液体，加入8ml增补的Earle培养液稀释，500g离心5min，弃上清，加入Earle培养液0.5ml重新悬浮细胞，用于精子密度或功能的评估。
(2) 非连续密度梯度法：取一个无菌的15ml锥底离心管，加入3ml 80%的Percoll。轻轻加入3ml 40%的Percoll于80%的Percoll液面上，小心操作，不要打乱两种液体的界面。轻轻加入1-2ml精液于梯度溶液上，500g离心20min，弃上清，将管底的精子团重新悬浮于5-10ml的Earle培养液中，500g离心5min，弃上清，加入1mlEarle培养液，重新悬浮。
3、将含有精子细胞的离心管置于37℃含5%CO2、95%空气的细胞培养箱中孵育1h；如无上述培养箱，可将离心管密封加盖，置于37℃的普通培养箱内孵育；在孵育过程中，大多数活动的精子从精浆中游离到覆盖上面的培养液内。
4、500g离心精子悬液5min，使精子细胞密度接近于10×10⁶/ml，将精子重悬于0.5-1ml精子细胞BWW培养基中，并在37℃含5%CO2、95%空气的细胞培养箱内孵育18-24h；如无上述培养箱，可将离心管密封加盖，置于37℃的普通培养箱内孵育。注意：在孵育过程中将试管20°倾斜。
注意事项：
1、注意无菌操作，尽量避免污染。
2、如无Earle培养液和增补的Earle培养液，可用精子细胞BWW培养基代替。
3、试剂开封后请尽快使用，以防影响后续实验效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Normal semen is a mixture composed of secretions from the testes and epididymis with suspended spermatozoa, combined with secretions from the prostate, seminal vesicles, and bulbourethral glands upon ejaculation. The final ejaculated mixture is a viscous liquid. There are numerous methods for sperm analysis, among which culture-based detection is applicable.
BWW Medium for Sperm Cells, also known as capacitating medium, is mainly composed of a series of salt ions, phenol red, glucose, sodium pyruvate, BSA, and antibiotics. Each 1000 mL of BWW Medium contains 100,000 units of penicillin sodium salt and 100 mg of streptomycin. As a common nutrient solution designed for capacitation treatment of sperm cells from a wide range of animals and humans, its standardized composition is based on the research published by the Biggers-Whitten-Whittingham (BWW) group in 1971, making it suitable for processing sperm cells from various animals and humans. This reagent is subjected to sterile treatment. For research use only. Not intended for clinical diagnosis or other purposes.
Materials to Be Prepared by Users
Sterile centrifuge tubes, Centrifuge, Cell culture incubator
Operating Procedures (For Reference Only)
1. Place the clean semen sample at room temperature for 30-60 minutes to allow complete liquefaction.
2. Sperm cell preparation (for reference only, non-mandatory step)
(1) Swim-up method
Take a sterile 15 mL conical-bottom centrifuge tube and add 1 mL of liquefied semen. Gently overlay 1.2 mL of Earle’s medium on top of the semen. Incline the tube at a 45° angle and incubate at 37°C for 1 hour. Slowly erect the tube and aspirate the top 1 mL of the liquid. Dilute the aspirated liquid with 8 mL of supplemented Earle’s medium, centrifuge at 500×g for 5 minutes, discard the supernatant, and resuspend the cells with 0.5 mL of Earle’s medium for the evaluation of sperm density or function.
(2) Discontinuous density gradient method
Take a sterile 15 mL conical-bottom centrifuge tube and add 3 mL of 80% Percoll. Gently overlay 3 mL of 40% Percoll on top of the 80% Percoll layer; handle with care to avoid disturbing the interface between the two solutions. Gently overlay 1-2 mL of semen on top of the gradient solution, centrifuge at 500×g for 20 minutes, and discard the supernatant. Resuspend the sperm pellet at the bottom of the tube in 5-10 mL of Earle’s medium, centrifuge at 500×g for 5 minutes, discard the supernatant, and resuspend the cells with 1 mL of Earle’s medium.
3. Incubate the centrifuge tube containing sperm cells in a cell culture incubator with 5% CO₂ and 95% air at 37°C for 1 hour. If such an incubator is unavailable, seal the tube with a cap and incubate in a conventional incubator at 37°C. During incubation, most motile sperm will swim out of the seminal plasma into the overlying culture medium.
4. Centrifuge the sperm suspension at 500×g for 5 minutes to adjust the sperm density to approximately 10×10⁶cells/mL. Resuspend the sperm in 0.5-1 mL of BWW Medium for Sperm Cells, and incubate the suspension in a cell culture incubator with 5% CO₂ and 95% air at 37°C for 18-24 hours. If such an incubator is unavailable, seal the tube with a cap and incubate in a conventional incubator at 37 °C. (Note: Incline the tube at a 20° angle during incubation.)
Precautions
1. Ensure aseptic operation and minimize the risk of contamination.
2. If Earle’s medium and supplemented Earle’s medium are unavailable, they can be replaced with BWW Medium for Sperm Cells.
3. Use the reagent as soon as possible after opening to prevent adverse effects on subsequent experimental results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
精子细胞BWW培养基 | 获能培养液
英文别名
BWW Medium for Sperm Cells | Capacitation Medium
规格或纯度
BioReagent,无菌,用于细胞培养
稳定性与储存
Store at -20℃ long term (12 months).
英文名称
BWW Medium for Sperm Cells (Sterile)
储存条件
-20°C储存
运输条件
超低温运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0434179 |
分析证书 | S1509606 |
技术文档和文章
溶液计算器
常见问题
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
什么是「用于细胞培养」？
「用于细胞培养」表示该产品经过加工和检测，适用于细胞培养和组织工程。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于?20 °C条件下保存。需冷冻保存以维持规定的有效期。
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