计算溶液所需的质量、体积或浓度。
精子核DNA染色液 (AO法)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
2-8°C储存,避光,室温。低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
正常情况下与精核DNA结合的碱性蛋白(核蛋白)将经历从组蛋白到鱼精蛋白的自然成熟过程，这种成熟后的鱼精蛋白对精子基因(DNA)具有特殊保护作用，组蛋白被鱼精蛋白逐渐取代的过程，称之为精子核蛋白组型转换，这种组型转换具有重要的生理意义。精子核携带着全部来自父方的遗传信息，这些基因必须在受精后才能开始表达，受精前精子基因在鱼精蛋白的特殊保护下紧密浓集，无任何DNA转录作用。但当核蛋白组型转换异常可引起男性不育或胚胎早期夭折流产，其机理为：①精子DNA不稳定且易受损伤而难以受孕；②一旦受精，由于核蛋白组型异常，精子核不能正常解聚，从而影响了雌雄原核的融合；③胚胎不能正常发育，造成胚胎夭折而流产。
正常精子核约占其头部的65％，由结合蛋白的DNA构成，精子核DNA染色液(AO法)作用原理是荧光染料吖啶橙与双链DNA结合发出绿色荧光，与单链DNA结合后可发出红色或黄色荧光，通常有双链DNA的精子才能有受精能力。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
产品组分及储存条件：
| S1511580 | Component | 20T | Storage |
| S1511580A | 洗涤液 | 100mL | RT |
| S1511580B | 固定液 | 50mL | RT |
| S1511580C | AO染色液 | 1mL | 2-8℃. Store in the dark. |
| S1511580D | AO Buffer | 5mL | 2-8℃ |
| S1511580E | 抗荧光淬灭封片剂 | 5mL | 2-8℃ |
自备材料：
1、蒸馏水、新鲜精液样本
2、EP管或离心管、恒温箱、防脱载玻片
操作步骤(仅供参考)：
1、取新鲜精液标本，置于恒温箱37°C或常温放置，至完全液化。
2、取上述液化精液0.2~0.5ml置于EP管，再加入1~1.5ml洗涤液，用吸管或移液器反复吹打数次，室温2000rpm离心5~10min，弃去上清液，保留管底精子沉淀团，重复操作1~2次。
3、向精子沉淀加入洗涤液约0.05~0.2ml，制成混合精子悬液。
4、取上述已制备好的精子悬液15~100μl，均匀涂布于防脱载玻片，自然干燥。
5、在涂片区内滴加固定液2~3滴，室温固定10~15min，蒸馏水稍洗，并甩去多余水分。
6、配制AO染色工作液：按AO染色液：AO Buffer=1：4的比例混合，即为AO染色工作液，注意该工作液应即配即用，不宜久置。
7、在涂片区内滴加AO染色工作液2~4滴，室温染色5min，蒸馏水稍洗，并甩去多余水份。
8、吹干或晾干玻片，滴加2~4滴抗荧光淬灭封片剂，荧光显微镜下观察。
染色结果：
核DNA：绿色荧光
注意事项：
1、蒸馏水冲洗载玻片时要注意控制水流速度，以免洗脱涂片区内的精子。
2、甩去多余水分，应防止涂片过于干燥。
3、配制的AO染色工作液应即配即用，不宜久置，否则荧光会衰减，如果荧光明显衰减可提高AO染色液：AO Buffer=1：4配制比例，以达到较好的效果。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
Under normal conditions, the basic proteins (nuclear proteins) bound to sperm nuclear DNA undergo a natural maturation process from histones to protamines. The mature protamines exert a special protective effect on sperm genes (DNA). The gradual replacement of histones by protamines is referred to as sperm nucleoprotein transition, which has important physiological significance. The sperm nucleus carries all the paternal genetic information, and these genes can only be expressed after fertilization. Before fertilization, sperm genes are tightly condensed under the special protection of protamines, with no DNA transcription activity. However, abnormal nucleoprotein transition can lead to male infertility, early embryonic death or miscarriage. The mechanisms are as follows:① Sperm DNA becomes unstable and susceptible to damage, resulting in difficulty in fertilization;② Once fertilized, the sperm nucleus cannot decondense normally due to abnormal nucleoprotein composition, thus interfering with the fusion of male and female pronuclei;③ The embryo fails to develop normally, leading to embryonic death and miscarriage. In normal sperm, the nucleus accounts for approximately 65% of the sperm head and is composed of DNA bound with proteins.Sperm Nuclear DNA Staining Solution (AO Method)Its working principle is that the fluorescent dye acridine orange emits green fluorescence when bound to double-stranded DNA, and red or yellow fluorescence when bound to single-stranded DNA. Generally, only sperm with double-stranded DNA are capable of fertilization. This reagent is for research use only and is not intended for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
| S1511580 | Component | 20T | Storage |
| S1511580A | Wash Buffer | 100mL | RT |
| S1511580B | Fixative Solution | 50mL | RT |
| S1511580C | AO Stain | 1mL | 2-8℃. Store in the dark. |
| S1511580D | AO Buffer | 5mL | 2-8℃ |
| S1511580E | Anti-Fade Mounting Medium | 5mL | 2-8℃ |
Materials Required:
1. Distilled water, fresh semen sample
2. EP tubes or centrifuge tubes, incubator, adhesive microscope slides
Protocol (For Reference Only):
1. Place the fresh semen sample in an incubator at 37 °C or at room temperature until completely liquefied.
2. Transfer 0.2–0.5 mL of liquefied semen into an EP tube, add 1–1.5 mL wash buffer, and mix by repeated pipetting. Centrifuge at 2000 rpm for 5–10 min at room temperature, discard the supernatant, and retain the sperm pellet. Repeat 1–2 times.
3. Add 0.05–0.2 mL wash buffer to the sperm pellet to prepare a sperm suspension.
4. Apply 15–100 μL of the sperm suspension evenly onto an adhesive microscope slide and air-dry.
5. Add 2–3 drops of fixative solution onto the smear, fix at room temperature for 10–15 min, rinse briefly with distilled water, and decant excess water.
6. Prepare AO working solution: mix AO stain and AO buffer at a ratio of 1:4. Prepare fresh before use and do not store.
7. Add 2–4 drops of AO working solution onto the smear, stain at room temperature for 5 min, rinse briefly with distilled water, and decant excess water.
8. Blow-dry or air-dry the slide, add 2–4 drops of anti-fade mounting medium, and observe under a fluorescence microscope.
Staining Results:
Nuclear DNA: green fluorescence
Precautions:
1. When rinsing slides with distilled water, control the flow rate to avoid washing off sperm from the smear.
2. After decanting excess water, prevent the smear from becoming overly dry.
3. AO working solution must be prepared fresh and used immediately; otherwise, fluorescence will fade. If fluorescence is significantly reduced, maintain the ratio of AO stain:AO buffer = 1:4 for optimal results.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
规格
别名
精子核DNA染色试剂盒
英文别名
Sperm Nuclear DNA Stain
规格或纯度
BioReagent,生物染色剂,用于显微镜
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Sperm Nuclear DNA Staining Solution (AO Method)
储存条件
2-8°C储存,避光,室温
运输条件
低温运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 E2618502 |
分析证书 | S1511580 |
| ZJ26F0433802 |
分析证书 | S1511580 |
技术文档和文章
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于2–8 °C、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品如何运输？
本产品采用湿冰冷藏运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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