计算溶液所需的质量、体积或浓度。
苏丹Ⅲ酒精饱和溶液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
中性脂肪染色经常采用苏丹I、苏丹Ⅲ、苏丹IV、苏丹黑B、油红O法等。苏丹染料脂质染色的机理一般认为纯属物理学的脂溶作用和吸附作用。 苏丹类染料由于在脂质中的溶解度大于在有机溶剂的溶解度，所以染色时染料便从染液中转移到被染的脂质中去，使脂质呈现出染液的颜色。苏丹酒精饱和溶液主要用于显示组织器官的脂肪变性和类脂质的异常沉着，常发生于肝、肾、心等实质脏器的脂肪变性，细胞内出现多数中性脂肪滴；鉴别和诊断脂肪组织中所发生的肿瘤及其性质。
操作步骤(仅供参考)：
1、新鲜组织低温切片 ，一般20°C ~ -25°C。如样本为脂肪瘤，应调节到-30°C。
2、冰冻切片5~ 10um(6~ 8μm为佳) ,贴于载玻片上。
3、70%乙醇稍微浸洗 下。
4、入苏丹酒精饱和溶液浸染1-2min。
5、70%乙醇洗去多余染色液。
6、蒸馏水浸洗1min。
7、按实验具体要求进行 下游实验。
染色结果：
中性脂肪--橙红色
注意事项：
1、脂肪染色时标本不宜采用含有乙醇的固定液、也不宜用石蜡切片，需用冰冻切片。
2、在染色过程中必须防止染料发生沉淀。故切片入染液时应密封, 勿与流动空气相接触，避免溶液挥发时发生沉淀。
3、冰冻切片较易着色,复染时应避免过染。
4、苏丹染料容易褪色,应密闭保存。
5、为了您的安全和健康,请穿实验服并戴一次性手套操作。
6、本产品仅用于科研，严禁他用。
Common methods for neutral fat staining include Sudan I, Sudan III, Sudan IV, Sudan Black B, Oil Red O staining, etc. The mechanism of lipid staining with Sudan dyes is generally considered to be purely physical liposolubility and adsorption. Since Sudan dyes have a higher solubility in lipids than in organic solvents, the dyes transfer from the staining solution to the stained lipids during staining, rendering the lipids the color of the staining solution. Saturated Sudan alcoholic solution is mainly used to demonstrate fatty degeneration of tissues and organs and abnormal deposition of lipoids, which often occurs in parenchymal organs such as the liver, kidney and heart, with numerous neutral fat droplets appearing in cells; it is also used to identify and diagnose tumors occurring in adipose tissue and their properties.
Procedures (for reference only):
1. Prepare frozen sections of fresh tissue at a low temperature, generally -20°C ~ -25°C. For lipoma samples, the temperature should be adjusted to -30°C.
2. Cut frozen sections at 5~10 μm (6~8 μm is optimal), and mount them on glass slides.
3. Rinse briefly with 70% ethanol.
4. Immerse in saturated Sudan alcoholic solution for 1–2 min.
5. Wash away excess staining solution with 70% ethanol.
6. Rinse with distilled water for 1 min.
7. Perform downstream experiments according to specific experimental requirements.
Staining Result:
Neutral fat – orange-red
Precautions:
1. For fat staining, specimens should not be fixed with ethanol-containing fixatives or prepared as paraffin sections; frozen sections are required.
2. Dye precipitation must be prevented during staining. Therefore, sections should be sealed when immersed in the staining solution to avoid contact with circulating air, which can cause precipitation due to solvent volatilization.
3. Frozen sections stain easily; overstaining should be avoided during counterstaining.
4. Sudan dyes fade easily and should be stored in a sealed container.
5. For your safety and health, please wear a laboratory coat and disposable gloves during operation.
6. This product is for research use only; strictly prohibited for other purposes.
规格
别名
苏丹 Ⅲ 染液 | 苏丹 Ⅲ 乙醇饱和溶液
英文别名
Sudan III stain
规格或纯度
BioReagent, 生物染色剂, 用于显微镜
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Sudan III Alcohol Saturated Solution
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0838628 |
分析证书 | S1520411 |
| ZJ26F0434340 |
分析证书 | S1520411 |
技术文档和文章
溶液计算器
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