脂质 (Lipid) 是中性脂肪、类脂及其衍生物的总称，其共同的物理特性是不溶于水，易溶于有机溶剂 (如乙醇、乙醚等)。人体的脂肪主要有两种：1、储存脂肪，如中性脂肪，主要分布于皮下、肾、胰腺等部位。2、结构脂肪，如类脂 (磷脂、糖脂、胆固醇等)，主要分布于细胞内。中性脂肪 (Neutral fat) 是由三分子脂肪酸和一分子甘油组成的脂类，呈中性。中性脂肪是储存能量的方式之一，在氧化时释放出能量。在正常情况下，除脂肪细胞外，其他细胞在光学显微镜下几乎看不到脂滴，如果细胞质内出现大量脂滴即为脂肪变性，常见于肝细胞、心肌细胞、肾曲管上皮细胞等。中性脂肪染色经常采用苏丹Ⅱ、苏丹Ⅲ、苏丹Ⅳ、苏丹黑B、油红O法等。苏丹染料脂质染色的机理一般认为纯属物理学的脂溶作用和吸附作用。苏丹类染料由于在脂质中的溶解度大于在有机溶剂的溶解度，所以染色时染料便从染液中转移到被染的脂质中去，使脂质呈现出染液的颜色。

苏丹Ⅳ酒精饱和溶液主要用于显示组织器官的脂肪变性和类脂质的异常沉着，常发生于肝、肾、心等实质脏器的脂肪变性，细胞内出现多数中性脂肪滴；鉴别和诊断脂肪组织中所发生的肿瘤及其性质。标本不采用含有乙醇的固定液 (如需要固定可采用10% 福尔马林)，也不采用石蜡切片，需用冰冻切片或碳蜡切片。

自备材料：

1、载玻片

2、70%乙醇

3、蒸馏水

操作步骤 (仅供参考)：

1、新鲜组织低温切片。如样本为脂肪瘤，应调节温度。

2、冰冻切片，贴于载玻片上。

3、乙醇稍微浸洗一下。

4、放入苏丹 IV 酒精饱和溶液，浸染。

5、乙醇洗去多余染色液。

6、蒸馏水浸洗。

7、按实验具体要求进行下游实验。

染色结果：

中性脂肪：猩红色

注意事项：

1、脂肪染色时标本不宜采用含有乙醇的固定液、也不宜用石蜡切片，需用冰冻切片。

2、在染色过程中必须防止染料发生沉淀。故切片入染液时应密封，勿与流动空气相接触，避免溶液挥发时发生沉淀。

3、冰冻切片较易着色，复染时应避免过染。

4、苏丹染料容易褪色，应密闭保存。

5、为了您的安全和健康，请穿实验服并戴一次性手套操作。

6、本产品仅由于科研，严禁他用。

Lipids are a general term for neutral fats, lipoids, and their derivatives. Their common physical property is being insoluble in water but easily soluble in organic solvents (such as ethanol, ether, etc.). There are mainly two types of fats in the human body: Storage fats, such as neutral fats, which are mainly distributed in subcutaneous tissues, kidneys, pancreas, and other parts. Structural fats, such as lipoids (phospholipids, glycolipids, cholesterol, etc.), which are mainly distributed inside cells. Neutral fats (Neutral fat) are lipids composed of three molecules of fatty acids and one molecule of glycerol, and they are neutral. As one of the ways to store energy, neutral fats release energy when oxidized. Under normal circumstances, except for fat cells, lipid droplets are barely visible in other cells under an optical microscope. If a large number of lipid droplets appear in the cytoplasm, it is called fatty degeneration, which is common in hepatocytes, cardiomyocytes, renal tubular epithelial cells, and other cells. Common methods for neutral fat staining include Sudan II, Sudan III, Sudan IV, Sudan Black B, and Oil Red O staining. The mechanism of lipid staining with Sudan dyes is generally considered to be purely physical fat solubility and adsorption. Since Sudan dyes have higher solubility in lipids than in organic solvents, the dyes transfer from the staining solution to the lipids being stained during staining, making the lipids show the color of the staining solution.

Sudan IV Alcohol Saturated Solution is mainly used to show fatty degeneration of tissues and organs and abnormal deposition of lipoids. It is often used for fatty degeneration of parenchymal organs such as the liver, kidneys, and heart, where a large number of neutral lipid droplets appear in cells. It is also used to identify and diagnose tumors occurring in adipose tissue and their properties. For specimens, fixatives containing ethanol should not be used (10% formalin can be used if fixation is required), and paraffin sections should not be used either; frozen sections or carbowax sections must be used.

Self-Prepared Materials

1. Glass slides

2. 70% ethanol

3. Distilled water

Operating Procedures (for reference only)

1. Prepare cryosections of fresh tissues. If the sample is a lipoma, adjust the temperature accordingly.

2. Mount the frozen sections on glass slides.

3. Rinse slightly with ethanol.

4. Immerse the sections in Saturated Alcohol Solution of Sudan IV for staining.

5. Wash off the excess staining solution with ethanol.

6. Rinse with distilled water.

7. Conduct subsequent experiments according to specific experimental requirements.

Staining Results

Neutral fats: Scarlet red

Precautions

1. For fat staining, specimens should not use fixatives containing ethanol or paraffin sections; frozen sections must be used.

2. Precipitation of the dye must be prevented during the staining process. Therefore, when placing sections into the staining solution, the container should be sealed to avoid contact with flowing air, preventing precipitation caused by solution volatilization.

3. Frozen sections are easier to stain, so over-staining should be avoided during counterstaining.

4. Sudan dyes fade easily and should be stored in a sealed container.

5. For your safety and health, wear a lab coat and disposable gloves during operation.

6. This product is for scientific research only and must not be used for other purposes.

规格

规格或纯度

BioReagent,用于显微镜,生物染色剂

稳定性与储存

Store at room temperature long term (12 months). Store in the dark.

英文名称

Sudan IV Alcohol Saturated Solution

储存条件

避光,室温

运输条件

常规运输

产品线

生物产品

名称和识别符

EC号

201-635-8

分子类型

生物试剂/缓冲液

Isomeric SMILES

CC1=CC=CC=C1N=NC2=CC(=C(C=C2)N=NC3=C(C=CC4=CC=CC=C43)O)C

NSC Number

10472

分子量

380.44

Beilstein号

709018

技术文档

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高级数据

化学和物理性质

敏感性

Light-sensitive

熔点

199°C

安全和危险性（GHS）

象形图

GHS07

信号词

Warning

危险声明

H315: 引起皮肤刺激

H319: 引起严重眼睛刺激

预防措施声明

P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。

P280: 戴防护手套/穿防护服/戴防护眼罩/戴防护面具。

P302+P352: 如皮肤沾染：用水充分清洗。

P321: 特殊处理（请参阅此标签上的...）。

P264: 处理后要彻底洗手。

P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。

P264+P265: 处理后彻底洗手[和…]。不要触摸眼睛。

P337+P317: 如果眼睛刺激持续：寻求医疗帮助。

P332+P317: 如果出现皮肤刺激：请寻求医疗帮助。

WGK Germany

RTECS

QL5775000

Class

Merck Index

8393

个人防护装备

dust mask type N95 (US),Eyeshields,Gloves

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批号(Lot Number)	证书类型	货号
ZJ25F1230651	分析证书	T1507851

技术文档和文章

t1507851_苏丹iv酒精饱和溶液.产品说明书-revised.pdf

t1507851 _sudan iv alcohol saturated solution product manual.pdf

血液细胞化学染色的原理、分类与临床实验应用

目录：技术文章

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PAS染色血液细胞化学染色骨髓涂片外周血涂片过氧化物酶染色苏丹黑B染色非特异性酯酶染色特异性酯酶染色中性粒细胞碱性磷酸酶铁染色白血病鉴别

Principles, Classification, and Clinical Laboratory Applications of Hematologic Cytochemical Staining

目录：技术文章

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PAS Staining Sudan Black B staining Hematologic Cytochemical Staining Bone Marrow Smear Peripheral Blood Smear Peroxidase Staining Nonspecific Esterase Staining Specific Esterase Staining Neutrophil Alkaline Phosphatase Iron Staining Leukemia Differentiation

常见脂质染色方法的原理、适用场景与结果判读

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BODIPY 组织化学染色胆固醇油红O 尼罗红脂质染色苏丹黑B 苏丹Ⅲ 苏丹Ⅳ 菲利平锇酸中性脂滴髓鞘脂质沉积

Principles, Applicable Scenarios, and Result Interpretation of Common Lipid Staining Methods

目录：技术文章

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BODIPY Cholesterol Oil Red O Nile Red lipid staining Sudan Black B Sudan III Sudan IV filipin osmium tetroxide neutral lipid droplets myelin lipid deposition histochemical staining

偶氮类染料型指示剂的结构特征、变色机制与应用选择

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甲基红刚果红染料型指示剂偶氮染料甲基橙橙黄G 铬黑T PAN PAR 钙镁试剂络合滴定金属显色剂组织染色

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植物组织和细胞壁染色方法：亮绿、番红、固绿与复合染色体系选择

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亮绿固绿植物组织染色细胞壁染色番红复合染色木质化维管束

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细胞染色方法总结

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Summary of Cell Staining Methods

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Annexin V Cell staining Hoechst Apoptosis detection Trypan Blue Crystal Violet cell viability JC-1 PI tissue staining Safranin Calcein-AM nuclear staining live-cell staining Eosin Sudan staining

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