产品形式:冷冻干粉,20mM 磷酸钾缓冲液,pH6.5;
产品介绍:
链霉亲和素是一种来源于阿维丁链霉菌(streptomyces avidinii)的生物素结合蛋白质,其非特异性结合远比亲和素低,链霉亲和素蛋白具有在中性pH值几乎没有净电荷,不含有碳水化合物等更有利的化学性质,所以它被广泛用来作为抗生物素蛋白的替代品。重组链霉亲和素是一种四聚体蛋白质,其分子量约为 53 kDa,由四个约 13 kDa的亚基组成。每个亚基都包含一个生物素结合位点,并有六个酪氨酸残基。纯化的重组链霉亲和素可用于检测生物素化抗体和其他探针的各种标准检测方法,包括免疫印迹、酶联免疫吸附测定、免疫组织化学和荧光成像
包装以蛋白含量计
Source:E. coli
Molecular weight:13.3kDa for monomer, 53kDa for tetramer
Accession:P22629 Ala37- Ser163 with an N-terminal Met
Protein amino acid sequence:AEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAAS
Purity:≥98% by SDS-PAGE or HPLC
Formulation:Lyophilized in 20 mM potassium phosphate buffer pH 6.5.
Biotin binding activity >17U/mg rSA protein(one unit binds 1µg D-biotin)
Endotoxin level:<0.01EU/ug rSA protein
Advantage:
1)high biotin binding activity
2)no extra amino sequence (tag free)
3)high purity
Product Form: Lyophilized powder, 20 mM potassium phosphate buffer, pH 6.5.
Product Application:
Streptavidin is a biotin-binding protein that was originally isolated from Streptomyces avidinii. Streptavidin is superior to avidin because it has almost no net charge at neutral pH and does not contain carbohydrate and exhibits lower level of non-specific binding. Recombinant streptavidin is a tetrameric protein with a molecular weight of approximately 53kDa, consisting of four subunits of approximately 13kDa. Each subunit contains a single biotin-binding site and has six tyrosine residues. The purified recombinant streptavidin can be applied in detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western Blotting, ELISA, immunohistochemistry and fluorescence imaging.:
Streptavidin is a biotin-binding protein that was originally isolated from Streptomyces avidinii. Streptavidin is superior to avidin because it has almost no net charge at neutral pH and does not contain carbohydrate and exhibits lower level of non-specific binding. Recombinant streptavidin is a tetrameric protein with a molecular weight of approximately 53kDa, consisting of four subunits of approximately 13kDa. Each subunit contains a single biotin-binding site and has six tyrosine residues. The purified recombinant streptavidin can be applied in detection of biotinylated antibodies and other probes in a variety of standard assay methods, including Western Blotting, ELISA, immunohistochemistry and fluorescence imaging.
Packaging based on protein content