本产品将链霉亲和素修饰在葡聚糖磁珠表面，通过链霉亲和素-生物素（SA-Biotin）系统的极高亲和力，高效结合生物素化抗体、核酸、蛋白等配体，非特异性吸附低，可广泛应用于细胞分选、分子诊断、免疫诊断等领域。本产品采用超顺磁性磁珠，磁响应速度快，适配自动化设备进行高通量操作；磁珠表面包覆葡聚糖分子，具有较高的生物相容性和亲水性，优良的悬浮稳定性和再分散性，更利于方便、快捷地捕获目标分子，确保反应的一致性。
| 产品号 |
产品名称 |
浓度 |
粒径 |
表面电位 |
是否无菌处理 |
| S1522863-A1 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
50 nm |
＜ -30 mV |
否 |
| S1522863-A2 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
50 nm |
＜ -30 mV |
是 |
| S1522863-B1 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
100 nm |
＜ -30 mV |
否 |
| S1522863-B2 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
100 nm |
＜ -30 mV |
是 |
| S1522863-C1 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
200 nm |
＜ -30 mV |
否 |
| S1522863-C2 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
200 nm |
＜ -30 mV |
是 |
| S1522863-D1 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
300 nm |
＜ -30 mV |
否 |
| S1522863-D2 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
300 nm |
＜ -30 mV |
是 |
| S1522863-E1 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
400 nm |
＜ -30 mV |
否 |
| S1522863-E2 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
400 nm |
＜ -30 mV |
是 |
| S1522863-F1 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
500 nm |
＜ -30 mV |
否 |
| S1522863-F2 |
链霉亲和素葡聚糖磁珠 |
10 mg/mL |
500 nm |
＜ -30 mV |
是 |
使用说明
以链霉亲和素葡聚糖磁珠捕获细胞为例，可根据不同的实验需求进行调整。
一、自备仪器与试剂
1. 仪器
磁力架：可选用2孔磁力架、5 mL磁力架、多功能磁力架等，需与实验所用离心管/流式管规格适配，确保磁分离效果稳定。
2. 适配耗材根据选用的磁力架及实验需求，自备对应规格耗材，包括但不限于：2 mL离心管、5 mL离心管、15 mL离心管、50 mL离心管、5 mL流式管。
3. 所需试剂
生物素化抗体：可选用商品化试剂，或自行标记（标记参考步骤：取100 μg抗体，用100 μL 0.01 M、pH9.0的CB缓冲液稀释混匀，涡旋条件下加入3 μg NHS-PEG4-生物素，置于37℃摇床反应45 min；反应完成后，采用10 KD超滤管、10 mM、pH7.4的PBS进行超滤洗涤，并重悬抗体备用）。
分选结合缓冲液：配方为0.01 M PBS + 2 mM EDTA + 0.5% BSA（或2% FBS），调节pH至7.2，经0.22 μm滤膜过滤后，于2-8℃冷藏保存备用；注意：BSA和FBS中应避免含有生物素，防止干扰实验。
分离缓冲液：配方为0.01 M PBS + 0.5% BSA + 2 mM EDTA，调节pH至7.2，经0.22 μm滤膜过滤后，于2-8℃冷藏保存备用。
流式抗体：根据具体实验目的、细胞类型，自行选择适配的流式抗体。
链霉亲和素（SA）磁珠：实验核心试剂，使用前需按要求进行预清洗处理。
二、实验准备
1. SA磁珠预清洗
SA磁珠使用前需彻底混匀，可采用振荡、涡旋或水浴超声方式，确保磁珠均匀分散；取实验所需用量的磁珠置于离心管中，加入1 mL分选结合缓冲液，将离心管置于适配磁力架上，待上清液完全澄清后，弃去上清；重复上述清洗步骤2次，最后用与初始磁珠体积相同的分选结合缓冲液重悬磁珠，保持SA磁珠浓度不变，备用。
2. 耗材与试剂无菌处理
根据实验目的，确定是否需要使用无菌级耗材和试剂；若需无菌操作，所有耗材、试剂需提前进行无菌处理，避免杂菌污染影响实验结果。
3. 细胞准备
(1) 若初始样本为组织：需按照常规实验方法，将组织处理成单细胞悬液，去除组织碎片，确保细胞活性。
(2) 若初始样本为外周血：需采用合适的方法（如Ficoll密度梯度离心法），从外周血中分离获取PBMC细胞，洗涤后备用。
(3) 细胞重悬：采用分选结合缓冲液，将上述处理好的细胞重悬至浓度为1×10⁸个/mL，置于2-8℃冷藏备用，避免细胞死亡或聚集。
三、磁性分选步骤（以单次分离1×10⁷个细胞为例）
1. 抗体孵育：取100 μL 1×10⁸个/mL的细胞悬液，缓慢加入离心管底部（避免从管壁加入，防止细胞吸附损失）；加入2 μL生物素化抗体，轻轻混匀后，置于2-8℃孵育10-15 min。
注：生物素化抗体用量需参考抗体说明书推荐用量，可根据实际细胞量按比例增减。
2. 可选洗涤步骤：向细胞悬液中加入1 mL分选结合缓冲液，于4℃、800 rpm/min条件下离心5 min，弃去上清；加入80 μL分选结合缓冲液，轻柔重悬细胞。
注：若步骤1中生物素化抗体用量已优化，无多余抗体残留，可直接进行步骤3，无需洗涤。
3. 磁珠结合：向细胞悬液中加入10-20 μL预清洗好的SA磁珠，轻轻混匀后，室温孵育3-5 min。
注：SA磁珠与细胞的孵育时间可根据实验需求适当延长，确保结合充分。
4. 第一次磁分离：加入1 mL分离缓冲液，轻柔重悬细胞与磁珠的混合液；将离心管置于适配磁力架上，磁分离3 min，直至上清液完全澄清；用移液枪吸取上清液至干净的离心管中，标记为管1（阴选细胞初步收集管）。 注：吸取上清时需小心操作，避免混入磁珠；必要时可在管底残留少量上清，防止磁珠被误吸。
5. 重复洗涤与磁分离：向原离心管中加入1 mL分离缓冲液，轻柔重悬结合磁珠的细胞沉淀；再次置于磁力架上磁分离3 min，待上清澄清后，将上清液收集至管1中；重复此洗涤-收集步骤3次；最后，用适量分离缓冲液重悬离心管底部标记磁珠的细胞沉淀，标记为管2（阳选细胞管）。
注：① 吸取上清时避免混入磁珠，必要时保留少量管底上清；② 可根据实验需求，选择是否保留每次洗涤的上清至管1，保留上清可获取更多未结合磁珠的细胞。
6. 分选结果：管1中为阴选出的细胞（细胞表面无磁珠及生物素化抗体标记）；管2中为阳选出的细胞（细胞表面结合生物素化抗体及SA磁珠）；可根据实验目的，对管1、管2中的细胞进行后续实验操作。
注意事项
SA磁珠取用前必须充分混匀，防止磁珠沉降导致取用浓度不均；避免长时间超声处理，防止破坏磁珠表面结构，影响结合能力。
磁珠的使用和保存过程中，严禁冻融；需在2~8℃密封保存，禁止冷冻；每次使用前均需充分混匀。
所有缓冲液需提前配制并过滤，确保无杂质；冷藏保存时需密封，避免污染；使用前需恢复至合适温度（避免温度骤变影响细胞活性）。
细胞操作全程需轻柔，避免剧烈振荡，防止细胞破裂；磁分离过程中，确保离心管与磁力架适配，保证磁分离效果。
生物素化抗体的用量需严格控制，过量抗体可能导致非特异性结合，影响分选纯度；建议提前进行抗体用量优化实验。
This product features streptavidin immobilized on the surface of dextran magnetic beads. Leveraging the extremely high affinity of the streptavidin-biotin (SA-Biotin) system, it efficiently binds biotinylated antibodies, nucleic acids, proteins, and other ligands, with low non‑specific adsorption. It is widely applicable in cell sorting, molecular diagnostics, immunoassays, and related fields. The product utilizes superparamagnetic magnetic beads with fast magnetic responsiveness, making it compatible with automated equipment for high‑throughput operations. The bead surface is coated with dextran molecules, providing high biocompatibility and hydrophilicity, excellent suspension stability and redispersibility, facilitating convenient and rapid capture of target molecules and ensuring assay consistency.
| Product Code | Product Name | Concentration | Particle Size | Surface Zeta Potential | Sterile (Yes/No) |
|---|
S1522863-A1
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 50 nm | < -30 mV | No |
S1522863-A2
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 50 nm | < -30 mV | Yes |
S1522863-B1
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 100 nm | < -30 mV | No |
S1522863-B2
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 100 nm | < -30 mV | Yes |
S1522863-C1
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 200 nm | < -30 mV | No |
S1522863-C2
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 200 nm | < -30 mV | Yes |
S1522863-D1
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 300 nm | < -30 mV | No |
S1522863-D2
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 300 nm | < -30 mV | Yes |
S1522863-E1
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 400 nm | < -30 mV | No |
S1522863-E2
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 400 nm | < -30 mV | Yes |
S1522863-F1
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 500 nm | < -30 mV | No |
S1522863-F2
| Streptavidin Dextran Magnetic Beads | 10 mg/mL | 500 nm | < -30 mV | Yes |
Instructions for Use
The following protocol uses streptavidin dextran magnetic beads for cell capture as an example; adjustments can be made according to specific experimental requirements.
I. Self‑Prepared Instruments and Reagents
1. Instruments
Magnetic separator: e.g., 2‑position magnetic rack, 5 mL magnetic rack, multi‑function magnetic rack, etc. The separator must be compatible with the centrifuge tubes/flow tubes used in the experiment to ensure stable magnetic separation.
2. Compatible Consumables
Based on the chosen magnetic separator and experimental needs, prepare appropriate consumables, including but not limited to: 2 mL centrifuge tubes, 5 mL centrifuge tubes, 15 mL centrifuge tubes, 50 mL centrifuge tubes, and 5 mL flow tubes.
3. Required Reagents
Biotinylated antibody: Commercially available reagents or self‑labeled (labeling reference: take 100 μg antibody, dilute and mix with 100 μL 0.01 M CB buffer, pH 9.0; under vortexing, add 3 μg NHS‑PEG4‑biotin; incubate at 37°C for 45 min with shaking; after reaction, use a 10 KD ultrafiltration tube and 10 mM PBS, pH 7.4, to wash by ultrafiltration, then resuspend the antibody for use).
Sorting/binding buffer: 0.01 M PBS + 2 mM EDTA + 0.5% BSA (or 2% FBS), adjust pH to 7.2, filter through 0.22 μm membrane, store at 2‑8°C. Note: BSA and FBS should be biotin‑free to avoid interference.
Separation buffer: 0.01 M PBS + 0.5% BSA + 2 mM EDTA, adjust pH to 7.2, filter through 0.22 μm membrane, store at 2‑8°C.
Flow cytometry antibodies: Choose appropriate antibodies according to the experimental purpose and cell type.
Streptavidin (SA) magnetic beads: Core reagent; pre‑washing is required before use.
II. Experimental Preparation
1. Pre‑washing of SA Magnetic Beads
SA magnetic beads must be thoroughly mixed before use (by vortexing, shaking, or water bath sonication) to ensure uniform dispersion. Transfer the required volume of beads to a centrifuge tube, add 1 mL sorting/binding buffer, place the tube on an appropriate magnetic separator, wait until the supernatant is completely clear, then discard the supernatant. Repeat the washing step twice. Finally, resuspend the beads in sorting/binding buffer to the original bead volume, keeping the SA bead concentration unchanged, and set aside.
2. Sterile Handling of Consumables and Reagents
Depending on the experimental purpose, determine whether sterile‑grade consumables and reagents are required. If aseptic operation is needed, all consumables and reagents must be pre‑sterilized to avoid microbial contamination.
3. Cell Preparation
(1) If starting from tissue: Process the tissue into a single‑cell suspension according to standard methods, remove tissue debris, and ensure cell viability.
(2) If starting from peripheral blood: Isolate PBMCs using an appropriate method (e.g., Ficoll density gradient centrifugation), wash, and set aside.
(3) Cell resuspension: Resuspend the prepared cells in sorting/binding buffer to a concentration of 1×10⁸ cells/mL, store at 2‑8°C to avoid cell death or aggregation.
III. Magnetic Sorting Procedure (example for 1×10⁷ cells per separation)
Antibody incubation: Gently add 100 μL of the 1×10⁸ cells/mL suspension to the bottom of a centrifuge tube (avoid adding down the tube wall to prevent cell adhesion loss). Add 2 μL of biotinylated antibody, mix gently, and incubate at 2‑8°C for 10‑15 min.
Note: The amount of biotinylated antibody should follow the manufacturer’s recommendation and can be scaled proportionally according to the actual cell number.
Optional washing step: Add 1 mL sorting/binding buffer to the cell suspension, centrifuge at 4°C, 800 rpm for 5 min, discard the supernatant. Add 80 μL sorting/binding buffer and gently resuspend the cells.
Note: If the biotinylated antibody amount in step 1 has been optimized with no excess antibody remaining, proceed directly to step 3 without washing.
Bead binding: Add 10‑20 μL of pre‑washed SA magnetic beads to the cell suspension, mix gently, and incubate at room temperature for 3‑5 min.
Note: The incubation time can be extended if needed to ensure complete binding.
First magnetic separation: Add 1 mL separation buffer and gently resuspend the cell‑bead mixture. Place the tube on an appropriate magnetic separator and magnetically separate for 3 min until the supernatant is completely clear. Carefully transfer the supernatant to a clean centrifuge tube labeled Tube 1 (preliminary collection of negatively selected cells).
Note: Avoid aspirating beads; if necessary, leave a small amount of supernatant at the bottom to prevent bead uptake.
Repeat washing and magnetic separation: Add 1 mL separation buffer to the original tube, gently resuspend the bead‑bound cell pellet. Place again on the magnetic separator for 3 min, and after the supernatant clears, transfer the supernatant to Tube 1. Repeat this wash‑collection step three times. Finally, resuspend the bead‑labeled cell pellet at the bottom of the original tube with an appropriate volume of separation buffer; label this tube as Tube 2 (positively selected cells).
Note: ① Avoid aspirating beads; leave a small amount of supernatant if needed. ② Depending on the experiment, you may choose to keep or discard each wash supernatant; retaining it collects more unbound cells.
Sorting results: Tube 1 contains negatively selected cells (cells without beads or biotinylated antibody on the surface); Tube 2 contains positively selected cells (cells bound with biotinylated antibody and SA beads). Perform downstream experiments on Tube 1 and Tube 2 according to the experimental purpose.
Precautions
- SA magnetic beads must be thoroughly mixed before each use to prevent concentration unevenness due to settling. Avoid prolonged sonication to prevent damage to the bead surface structure and binding capacity.
- Do not freeze‑thaw the beads during use or storage. Store sealed at 2‑8°C, do not freeze. Mix well before each use.
- All buffers should be prepared in advance, filtered, and kept free of impurities. Store sealed at 2‑8°C; equilibrate to appropriate temperature before use (avoid sudden temperature changes that may affect cell viability).
- All cell handling should be gentle, avoiding vigorous shaking to prevent cell rupture. Ensure the centrifuge tube fits well on the magnetic separator for effective separation.
- The amount of biotinylated antibody must be strictly controlled; excess antibody may cause non‑specific binding and reduce sorting purity. Optimize antibody amount in advance if necessary.
ZJ26F0737824
危险品化学品经营许可证（带存储）