D2624428计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,-20°C储存,充氩。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
N-羟基琥珀酰亚胺（NHS）功能化的聚乙二醇（PEG-NHS） 是一种氨基（-NH₂）反应性PEG衍生物，可用于修饰蛋白质、多肽或任何带有可及氨基基团的表面。在pH 7~8.5条件下，NHS酯与伯胺基团反应形成稳定的酰胺键。 与其他PEG NHS酯衍生物相比，琥珀酰亚胺碳酸酯（SC）功能化的mPEG-NHS在水溶液中具有更优的反应活性和更高的稳定性。NHS酯与去质子化的伯胺反应，因此该反应需要中性至碱性的pH值才能进行。伯胺通过亲核攻击与NHS酯反应，同时NHS作为副产物释放出来。在水溶液中，NHS酯的水解反应与目标反应竞争，并且随着pH升高而加剧。
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
规格
规格或纯度
MW 2000 Da
英文名称
Succinimidyl acid PEG, mPEG-NHS
生化机理
Reaction Procedures:
Generally, a 10 to 50-fold molar excess of NHS PEG over the amount of amine-containing material results in sufficient conjugation.
Materials Required:
Conjugation buffer: Sodium bicarbonate 100 mM buffer, pH 8.5 or other amine-free buffer at pH 7-8.5.
PEG NHS stock solution: 100 mg in 1 mL conjugation buffer.
Washing solution: Distilled water or any aqueous buffer.
Reaction Steps:
Dissolve targeted materials in conjugation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add NHS PEG stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. 10~50 molar excess of PEG NHS needed for optimal conjugation; Allow mixture agitates at room temperature for 30~60 min at room temperature or 2 hours at 4 0C. Conjugates can be purified either by size exclusion chromatography or dialysis.
储存条件
避光,-20°C储存,充氩
运输条件
超低温运输
名称和识别符
分子类型
聚合物
技术文档
高级数据
化学和物理性质
溶解性
Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
敏感性
对湿度敏感；对光线敏感
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
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