计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,充氩。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
规格
规格或纯度
MW 20000 Da
英文名称
Succinimidyl acid PEG, mPEG-NHS
生化机理
In general, a molar excess of 10 to 50 times the amount of amine containing material in NHS PEG is sufficient for sufficient conjugation.
Required materials
Connection buffer: 100 mM sodium bicarbonate buffer with pH 8.5 or other amine free buffer with pH 7-8.5.
PEG NHS storage solution: Add 100mg to 1ml conjugate buffer.
Washing solution: distilled water or any aqueous buffer solution.
Reaction steps:
Dissolve the target material in conjugate buffer solution. Estimate the concentration of primary amine groups on the target material. Add NHS PEG stock solution to the target conjugated material, with a final concentration of at least 10 mg/mL. The optimal conjugation requires an excess of 10-50 moles of PEG NHS; Stir the mixture at room temperature for 30-60 minutes or at 40C for 2 hours. Conjugates can be purified by size exclusion chromatography or dialysis.
储存条件
-20°C储存,充氩
运输条件
超低温运输
名称和识别符
分子类型
聚合物
技术文档
高级数据
化学和物理性质
溶解性
Off-white/white solid or viscous liquid depends on molecule weight;Soluble in regular aqeous solution as well as most organic solvents;
敏感性
对光及湿度敏感
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 D2628379 |
分析证书 | S164317 |
| D2628380 |
分析证书 | S164317 |
| L2519358 |
分析证书 | S164317 |
| L2519368 |
分析证书 | S164317 |
| L2519394 |
分析证书 | S164317 |
| G2419484 |
分析证书 | S164317 |
| G2423012 |
分析证书 | S164317 |
| C2409105 |
分析证书 | S164317 |
| C2409106 |
分析证书 | S164317 |
| C2409107 |
分析证书 | S164317 |
| C2409108 |
分析证书 | S164317 |
技术文档和文章
溶液计算器
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