RH 414 [化学名称：N-(3-三乙基铵丙基)-4-[4-(4-二乙氨基苯基)丁二烯基]吡啶二溴化物] 是一种神经元示踪染料。该染料可用于监测神经元的膜电位变化、突触活动以及离子通道活性。
产品优势
1. 电位依赖性分布：探针的跨膜分布随膜电位变化而改变，并伴随荧光强度变化；
2. 毫秒级快速响应响：通过分子构象变化直接反映膜电位改变，应速度较快。
适用范围
用于监测细胞膜电位的快响应探针。
标准母液配制
表 1 不同质量 RH 414配制成指定浓度所需纯水溶解体积。
注：表中体积仅适用于母液配制；正式实验、生理缓冲体系的稀释配比请参照配套完整实验方案。
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 171.978 μL | 859.89 μL | 1.72 mL | 8.599 mL | 17.198 mL | | 5 mM | 34.396 μL | 171.978 μL | 343.956 μL | 1.72 mL | 3.444 mL | | 10 mM | 17.198 μL | 85.989 μL | 171.978 μL | 859.89 μL | 1.72 mL |
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RH 414 [N-(3-Triethylammoniumpropyl)-4-(4-(4-(diethylamino)phenyl)butadienyl)pyridinium dibromide] is a neuron-tracing dye. It is used for monitoring membrane potential, synaptic activity and ion channel activity of neurons.
Advantages
1. Potential-dependent distribution: The transmembrane distribution of the probe shifts with alterations in membrane potential, accompanied by corresponding changes in fluorescence intensity.
2. Millisecond-level fast response: It directly reflects membrane potential fluctuations via molecular conformational changes, featuring an ultra-rapid response rate.
Application
Fast-response fluorescent probe for monitoring cell membrane potential.
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of RH 414 to given concentration.
Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| Concentration \ Mass | 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg |
|---|
| 1 mM | 171.978 μL | 859.89 μL | 1.72 mL | 8.599 mL | 17.198 mL | | 5 mM | 34.396 μL | 171.978 μL | 343.956 μL | 1.72 mL | 3.444 mL | | 10 mM | 17.198 μL | 85.989 μL | 171.978 μL | 859.89 μL | 1.72 mL |
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