计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,生物活性,重组,高性能 级 提供 ≥95%(SDS-PAGE) 纯度,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Bacteriophage T4 Lysozyme breaks down bacterial cell walls. The enzyme attacks the peptidoglycans in the cell walls of bacteria and hydrolyzes the ß-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine. Specific activity of T4 lysozyme is significantly greater than egg white lysozyme when assayed with Micrococcus lysodeikticus and E. coli.
Protein Purity: ≥95% (SDS-PAGE)
Storage Buffer: 20 mM Tris-HCl, 250 mM NaCl, 1 mM DTT, 0.1mM EDTA, 50% Glycerol, pH 8.0.
Activity: 200 μl reaction in 50 mM Tris-HCl pH 8.0 buffer containing a Micrococcus lysodeikticussuspension and 1μg of T4 Lysozyme (E. coli) incubated for 20 minutes at 25°C results in a decrease of ≥0.05 in turbidity as determined by OD₄₅₀.
Applications:
Bacterial lysis for nucleic acid extraction
Bacterial lysis for recombinant protein extraction
Source:
A recombinant E. coli strain carrying the cloned bacteriophage T4 lysozyme gene.
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