肌纤维(Musclefiber)属于肌组织成分,由肌细胞组成,根据形态和功能特点,肌纤维可以分为平滑肌(又称横纹肌)、骨骼肌、心肌,肌纤维染色的方法有很多种,如丽春红法、苯胺蓝法、钨磷钨酸苏木素法等。目前使用磷钼酸作为媒染剂的苏木素较少,唯一受到大家认可的是McManus和Nowry采用的用于显示胶原和粗网状纤维的Thomas磷钼酸苏木素技术。
Thomas磷钼酸苏木素液主要由Thomas磷钼酸苏木素、Thomas分化液组成,主要用于胶原、粗网状纤维、嗜银细胞颗粒等染色。该试剂仅用于科研领域,不适用于临床诊断或其他用途。
产品组分及储存条件:
T1518604
| Component
| 2×100mL
| Storage
|
T1518604A
| Thomas 磷钼酸苏木素染色液
| 100mL
| RT. Store in the dark.
|
T1518604B
| Thomas 分化液
| 100mL
| RT |
自备材料:
系列乙醇、蒸馏水、二甲苯或环保脱蜡透明液、中性树胶
操作步骤(仅供参考):
1、常规组织固定脱水包埋,二甲苯或脱蜡透明液脱蜡,系列乙醇水化至水。
2、入Thomas磷钼酸苏木素染色液中染色2min,蒸馏水冲洗。
3、滴加Thomas分化液分化,蒸馏水快速冲洗,自来水冲洗2min。
4、95%乙醇和无水乙醇迅脱水。
5、常规脱水,二甲苯或脱蜡透明液透明,中性树胶封固。
染色结果:
胶原蛋白、粗网状纤维:紫色至黑色
嗜银细胞:黑色
细胞核:淡蓝色
注意事项:
1、避免采用重铬酸类固定液固定样本。
2、为了您的安全和健康,请穿实验服并戴一次性手套操作。
Muscle fibers are components of muscle tissue and are composed of muscle cells. According to their morphological and functional characteristics, muscle fibers can be classified into smooth muscle, skeletal muscle, and cardiac muscle (note: smooth muscle is not also known as striated muscle; only skeletal and cardiac muscle are striated). There are many staining methods for muscle fibers, such as Ponceau method, Aniline Blue method, Phosphotungstic Acid Hematoxylin method, etc. At present, there are few hematoxylin solutions using phosphomolybdic acid as a mordant, and the only widely recognized technique is the Thomas Phosphomolybdic Acid Hematoxylin method adopted by McManus and Nowry for demonstrating collagen and coarse reticular fibers.
Thomas Phosphomolybdic Acid Hematoxylin Staining Solution mainly consists of Thomas Phosphomolybdic Acid Hematoxylin and Thomas Differentiation Solution. It is mainly used for the staining of collagen, coarse reticular fibers, argentaffin cell granules, etc. This reagent is for research use only and not for clinical diagnosis or other purposes.
Product Components and Storage Conditions:
T1518604
| Component
| 2×100mL
| Storage
|
T1518604A
| Thomas Phosphomolybdic Acid Hematoxylin Staining Solution
| 100mL
| RT. Store in the dark.
|
T1518604B
| Thomas Differentiation Solution
| 100mL
| RT |
Materials Required (User-supplied):
Graded ethanol, distilled water, xylene or eco-friendly dewaxing clearing agent, neutral balsam
Protocol (For Reference Only):
1. Perform routine tissue fixation, dehydration and embedding. Dewax with xylene or dewaxing clearing agent, then hydrate to water through graded ethanol.
2. Stain in Thomas Phosphomolybdic Acid Hematoxylin Staining Solution for 2 min, then rinse with distilled water.
3. Differentiate with Thomas Differentiation Solution, rinse quickly with distilled water, then wash in running water for 2 min.
4. Dehydrate rapidly in 95% ethanol and absolute ethanol.
5. Complete routine dehydration, clear with xylene or dewaxing clearing agent, and mount with neutral balsam.
Staining Results:
Collagen fibers, coarse reticular fibers: Purple to black
Argentaffin cells: Black
Cell nuclei: pale Blue
Precautions:
1. Avoid using dichromate-based fixatives for specimen fixation.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.