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重组转谷氨酰胺酶(mTGase, no-tag)

重组转谷氨酰胺酶(mTGase, no-tag)

Name: 重组转谷氨酰胺酶(mTGase, no-tag)
SKU: T1526923
Availability: InStock

CAS: 80146-85-6 货号: T1526923 EC号: 616-952-0

20篇引用文献 COA

有货

级别和纯度: 生物活性 ? 重组 ? EnzymoPure™ ? ≥95%(SDS-PAGE) expressed in E.coli; ≥30 U/mg protein; 蛋白浓度: 见COA

Accession #

P81453

蛋白标签

No tag

表达系统

E. coli

生物活性

≥30 U/mg protein

规格

库存

价格

数量

0.1mg

T1526923-0.1mg

现货

¥999.90

0.5mg

T1526923-0.5mg

现货

¥2,999.90

1mg

T1526923-1mg

现货

¥3,999.90

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概述规格储存与运输技术文档高级数据质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章溶液计算器

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为什么选择此级别

EnzymoPure™,生物活性,重组级提供 ≥95%(SDS-PAGE) 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-80℃储存,避免反复冻融。特低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 20 篇同行评审文献引用。

概述

重组转谷氨酰胺酶(mTGase, no-tag) 是一种由E.coli重组表达的，来源于茂原链霉菌的高活性转谷胺酰胺酶。与动物来源的转谷氨酰胺酶相比，其具有分子量小，不依赖于钙离子的特点，可催化脱糖抗体第295位谷氨酰胺侧链与底物中的伯胺之间形成稳定的酰胺键。因为不需要额外的肽供体来促进偶联反应，只要目标分子上存在伯胺基团，即可发挥作用，具有很高的灵活性，因此经常被应用在ADC抗体制备中。

使用方法（仅供参考）

注：使用前，应将产品放在 2 - 8°C 条件下缓慢解冻，严禁采用室温或 37°C 快速解冻。

重组转谷氨酰胺酶(mTGase, no-tag)可催化脱糖抗体与氨基氨反应，生成的抗体-氨基氨化合物继而与含胺的药物连接子或反应性spacer反应，将其偶联至脱糖抗体。加量可根据具体工艺进行调整，与氨基氨反应的条件推荐为 25°C 或者 37°C 过夜（16-20h）。

产品应用

抗体的ADC偶联

注意事项

收到产品后尽快置于 -80°C 储存，避免反复冻融；

建议抗体脱糖后再进行偶联反应；

使用时请穿实验服并佩戴一次性手套；

本品不得直接用于临床诊断和治疗。

Recombinant transglutaminase (mTGase, no-tag) is a highly active microbial transglutaminase derived from Streptomyces mobaraensis and recombinantly expressed in E. coli. Compared with animal‑derived transglutaminases, it features a smaller molecular weight and is Ca²⁺‑independent. It catalyzes the formation of a stable amide bond between the glutamine side chain at position 295 of deglycosylated antibodies and primary amines in the substrate. Because it does not require an additional peptide donor to promote the coupling reaction, it can function as long as primary amine groups are present on the target molecule, offering high flexibility. Therefore, it is frequently used in the preparation of ADCs (antibody‑drug conjugates).

Usage Instructions (For Reference Only)

Note: Before use, the product should be slowly thawed at 2–8 °C. Rapid thawing at room temperature or 37 °C is strictly prohibited.

Recombinant transglutaminase (mTGase, no‑tag) catalyzes the reaction between deglycosylated antibodies and amino‑amine compounds. The resulting antibody‑amino‑amine conjugate then reacts with amine‑containing drug linkers or reactive spacers, thereby coupling them to the deglycosylated antibody. The amount of mTGase added can be adjusted according to the specific process. The recommended reaction conditions with amino‑amine compounds are 25 °C or 37 °C overnight (16–20 h).

Product Application

ADC conjugation of antibodies.

Precautions

Store the product at –80 °C as soon as possible after receipt. Avoid repeated freeze‑thaw cycles.

It is recommended to deglycosylate the antibodies before performing the conjugation reaction.

Please wear a lab coat and disposable gloves when handling.

This product is not intended for direct clinical diagnosis or treatment.