TBS-T为白色至类白色速溶颗粒，每袋TBS-T速溶颗粒可配制1L 1 × TBS-T缓冲液，操作简便，使用方便 。 TBS-T的主要成分是氯化钠、氯化钾、Tris 缓冲盐和Tween-20， 1 × TBS-T缓冲液中含有的氯化钠浓度为137 mM、氯化钾的浓度为2.7 mM，Tris缓冲盐的浓度为25 mM，Tween-20的含量为0.05%。配制成1 ×溶液后的 pH值为7.4±0.15@25℃。
TBS-T是一种在生物学上应用广泛的缓冲液，主要用于免疫组化、原位杂交、酶联免疫、免疫印迹实验中，常用作洗涤缓冲液，也常用于抗体的稀释以及封闭液的配制。
使用方法
1. 量取约600 ml的蒸馏水加入烧杯，并放置一个磁性搅拌子于烧杯中。
2. 将烧杯置于磁力搅拌器上，慢慢加入1袋TBS-T速溶颗粒的全部内容物，搅拌溶液直至完全溶解。
3. 向步骤2的TBS-T溶液中加入蒸馏水，定容至1 L，即为1 ×。
速溶颗粒配制说明
TBS-T appears as white to off-white instant granules. Each pouch of TBS-T instant granules is sufficient to prepare 1 L of 1× TBS-T buffer, featuring simple and convenient operation.The main components of TBS-T are sodium chloride, potassium chloride, Tris buffer salts and Tween-20. The 1× TBS-T buffer contains 137 mM sodium chloride, 2.7 mM potassium chloride, 25 mM Tris buffer salts and 0.05% Tween-20. The pH value of the prepared 1× solution is 7.4±0.15 at 25℃.
As a widely used biological buffer, TBS-T is mainly applied in immunohistochemistry, in situ hybridization (ISH), enzyme-linked immunosorbent assay (ELISA) and Western blotting. It is commonly used as a washing buffer, and also frequently for antibody dilution and blocking solution preparation.
Protocol
1. Measure approximately 600 mL of distilled water into a beaker, and place a magnetic stir bar in the beaker.
2. Place the beaker on a magnetic stirrer. Slowly add the entire contents of one pouch of TBS-T instant granules, and stir until completely dissolved.
3. Add distilled water to the TBS-T solution from Step 2 to a final volume of 1 L to obtain 1× working solution.
Preparation Instructions for Instant Granules
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