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概述
产品介绍：
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性能阐述：
在组织细胞的体外培养和原代细胞培养中的组织细胞分散（将组织块制备成单个细胞 悬液）以及传代细胞培养中，贴壁生长细胞的消化分散均要使用组织细胞消化液。消化液成分为胰蛋白酶，EDTA，酚红等，其功能主要是使细胞间的蛋白质（如细胞外基质）水解， 使组织或贴壁细胞分散成单个细胞，制成细胞悬液用于进一步的实验。
胰酶细胞消化液经过滤除菌，可以直接用于培养细胞和组织的消化。本产品具有方便快速、稳定安全、细胞状态好等特点。通常室温消化2分钟左右就可以消化下大多数贴壁细胞。酚红具有pH指示作用。
使用说明：
1、贴壁细胞的消化
1.1 吸去培养液，用无菌的PBS、Hanks液或无血清培养液洗涤细胞一次，以去除残余的血清。
1.2 加入少量胰蛋白酶消化液，盖过细胞即可，室温放置1-2分钟。不同的细胞消化时间有所不同，对于贴壁牢固的细胞可适当延长消化时间。
1.3 显微镜下观察，细胞明显收缩，并且肉眼观察培养器皿底部发现细胞的形态发生明显的变化；或者用枪吹打细胞发现细胞刚好可以被吹打下来。此时吸除消化液。加入含血清的细胞培养液，吹打下细胞，即可直接用于后续实验。
1.4 如果发现消化不足，可加入胰蛋白酶消化液重新消化。
1.5 如果发现细胞消化时间过长，未及吹打细胞，细胞已经有部分直接从培养器皿底部脱落，直接用胰酶细胞培养液把细胞全部吹打下来。1000-2000g离心1分钟，沉淀细胞，尽量去除胰酶细胞消化液后，加入含血清的完全培养液重新悬浮细胞，即可用于后续实验。
2、组织的消化
不同的组织需要消化的时间相差很大，通常以消化后可以充分打散组织为宜。
注意事项：
1、由于组织或细胞性质不同，实验人员应依据具体情况，确定最佳消化时间；消化细胞时间不宜过长，否则会影响细胞贴壁和生长状况。
2、 本产品不含抑菌剂，在使用过程中要特别注意无菌操作，避免消化液被微生物污染。
3、 不宜4℃长期保存，切忌反复冻融，小量使用时建议分装冻存。
4、 为了您的安全和健康，请穿戴实验服并戴一次性手套操作。
| Component No. | Component Name | Specification | Size | Storage |
| T753835‑TR‑100ml | Trypsin Cell Dissociation Solution | 0.25% Trypsin, with phenol red, with EDTA | 100 mL | Store at −20 °C. Avoid freeze/thaw cycles. |
| T753835‑WE‑100ml | Trypsin Cell Dissociation Solution | 0.25% Trypsin, without phenol red, without EDTA | 100 mL | Store at −20 °C. Avoid freeze/thaw cycles. |
| T753835‑WW‑100ml | Trypsin Cell Dissociation Solution | 0.25% Trypsin, with phenol red, without EDTA | 100 mL | Store at −20 °C. Avoid freeze/thaw cycles. |
Tissue‑cell dissociation solution is applied for tissue dissociation to prepare single‑cell suspensions in in‑vitro tissue culture and primary cell culture, as well as for detachment of adherent cells during subculture. Composed of trypsin, EDTA, phenol red and other components, it hydrolyzes intercellular proteins such as extracellular matrix, dissociating tissues or adherent cells into single‑cell suspensions for downstream experiments.
Sterilized by filtration, Trypsin Cell Dissociation Solution is ready‑to‑use for dissociation of cultured cells and tissues. It features convenient operation, fast processing, high stability, good safety and favorable cell viability. Most adherent cells can be dissociated within approximately 2 min at room temperature. Phenol red serves as a pH indicator.
Instructions for Use
1. Dissociation of adherent cells
1.1 Remove culture medium. Rinse cells once with sterile PBS, Hanks’ balanced salt solution or serum‑free medium to eliminate residual serum.
1.2 Add a minimal volume of trypsin dissociation solution to just cover the cell monolayer. Incubate at room temperature for 1‑2 min. Incubation time varies among cell types; extend incubation appropriately for strongly adherent cells.
1.3 Monitor under microscope: stop when cells exhibit obvious shrinkage, morphological alteration at the bottom of culture vessels, or can be gently dislodged by pipetting. Remove dissociation solution. Add serum‑supplemented complete medium and pipette to harvest cells for subsequent experiments.
1.4 If dissociation is insufficient, add fresh trypsin solution and repeat digestion.
1.5 If over‑digestion occurs and partial cells detach before pipetting, pipette to harvest all cells directly in trypsin solution. Centrifuge at 1000‑2000 g for 1 min to pellet cells. Remove trypsin supernatant as much as possible, then resuspend cell pellet in serum‑containing complete medium for downstream use.
2. Tissue dissociation
Digestion time varies greatly for different tissues. Terminate digestion when tissues can be fully triturated.
Precautions
1. Optimal digestion time should be determined empirically according to specific tissue or cell types. Over‑digestion impairs cell attachment and proliferation.
2. This product contains no bacteriostatic agents. Strict aseptic manipulation is required to prevent microbial contamination.
3. Do not store long‑term at 4 °C. Avoid repeated freeze‑thaw cycles. Aliquot for long‑term storage.
4. Wear laboratory coat and disposable gloves for personal protection during handling.
| Component No. | Component Name | Specification | Size | Storage |
| T753835‑TR‑100ml | Trypsin Cell Dissociation Solution | 0.25% Trypsin, with phenol red, with EDTA | 100 mL | Store at −20 °C. Avoid freeze/thaw cycles. |
| T753835‑WE‑100ml | Trypsin Cell Dissociation Solution | 0.25% Trypsin, without phenol red, without EDTA | 100 mL | Store at −20 °C. Avoid freeze/thaw cycles. |
| T753835‑WW‑100ml | Trypsin Cell Dissociation Solution | 0.25% Trypsin, with phenol red, without EDTA | 100 mL | Store at −20 °C. Avoid freeze/thaw cycles. |
Tissue‑cell dissociation solution is applied for tissue dissociation to prepare single‑cell suspensions in in‑vitro tissue culture and primary cell culture, as well as for detachment of adherent cells during subculture. Composed of trypsin, EDTA, phenol red and other components, it hydrolyzes intercellular proteins such as extracellular matrix, dissociating tissues or adherent cells into single‑cell suspensions for downstream experiments.
Sterilized by filtration, Trypsin Cell Dissociation Solution is ready‑to‑use for dissociation of cultured cells and tissues. It features convenient operation, fast processing, high stability, good safety and favorable cell viability. Most adherent cells can be dissociated within approximately 2 min at room temperature. Phenol red serves as a pH indicator.
Instructions for Use
1. Dissociation of adherent cells
1.1 Remove culture medium. Rinse cells once with sterile PBS, Hanks’ balanced salt solution or serum‑free medium to eliminate residual serum.
1.2 Add a minimal volume of trypsin dissociation solution to just cover the cell monolayer. Incubate at room temperature for 1‑2 min. Incubation time varies among cell types; extend incubation appropriately for strongly adherent cells.
1.3 Monitor under microscope: stop when cells exhibit obvious shrinkage, morphological alteration at the bottom of culture vessels, or can be gently dislodged by pipetting. Remove dissociation solution. Add serum‑supplemented complete medium and pipette to harvest cells for subsequent experiments.
1.4 If dissociation is insufficient, add fresh trypsin solution and repeat digestion.
1.5 If over‑digestion occurs and partial cells detach before pipetting, pipette to harvest all cells directly in trypsin solution. Centrifuge at 1000‑2000 g for 1 min to pellet cells. Remove trypsin supernatant as much as possible, then resuspend cell pellet in serum‑containing complete medium for downstream use.
2. Tissue dissociation
Digestion time varies greatly for different tissues. Terminate digestion when tissues can be fully triturated.
Precautions
1. Optimal digestion time should be determined empirically according to specific tissue or cell types. Over‑digestion impairs cell attachment and proliferation.
2. This product contains no bacteriostatic agents. Strict aseptic manipulation is required to prevent microbial contamination.
3. Do not store long‑term at 4 °C. Avoid repeated freeze‑thaw cycles. Aliquot for long‑term storage.
4. Wear laboratory coat and disposable gloves for personal protection during handling.
规格
产品名称
胰酶细胞消化液
别名
胰蛋白酶消化液 | 胰酶消化液
英文别名
Trypsin Solution | Trypsin | Trypsin-EDTA Solution
规格或纯度
BioReagent, 用于细胞培养, 无菌
分子类型
生物试剂/缓冲液
产品线
生物产品
储存与运输
物理形态
液体
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F1039733 |
分析证书 | T753835 |
| ZJ25F1129906 |
分析证书 | T753835 |
| ZJ25F0521879 |
分析证书 | T753835 |
| ZJ25F0521878 |
分析证书 | T753835 |
| ZJ25F0521877 |
分析证书 | T753835 |
技术文档和文章
此产品的引用文献
溶液计算器
常见问题
什么是「用于细胞培养」？
「用于细胞培养」表示该产品经过加工和检测，适用于细胞培养和组织工程。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于?20 °C条件下保存。避免反复冻融。建议按单次用量分装保存。
本产品如何运输？
本产品采用保温箱配冰袋运输。收货后请立即拆包，并按上述储存条件保存。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
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我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 for Cell culture 级别指南 → 查看 Sterile 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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