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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
尿素 (Urea) 又称碳酰胺 (carbamide)，是哺乳动物和某些鱼类体内蛋白质代谢分解的主要含氮终产物，也是目前含氮量最高的氮肥。尿素检测方法大致分为化学方法和酶学方法，后者被认为是间接方法，先经尿素酶 (脲酶) 分解尿素为铵离子，再根据波氏反应检测铵离子的生成量。
尿素（Urea）含量检测试剂盒（脲酶波氏比色法）检测原理：尿素酶水解尿素，生成氨与二氧化碳；氨在碱性条件下与苯酚类物质反应生成蓝色吲哚酚，吲哚酚生成量与尿素含量成正比。采用分光光度计测定 560 nm 处吸光度，可检测人体、动物的血浆、血清、尿液等样本中的尿素 (旧称尿素氮，BUN) 含量，尿液样本建议预处理后再检测。本试剂盒仅用于科研领域，不可应用于临床诊断及其他相关用途。
| 储运单元货号 | 储运单元名称 | 组分货号 | 组分名称 | 物理外观 | 100 T | 储存 | 单次测试使用量 |
| U1522046NT | 尿素 (Urea) 含量检测试剂盒 (脲酶波氏, 比色法) (常温组分) | U1522046F | ddH₂O | 无色液体 | 10 mL | 室温 | 请按说明配制 |
| U1522046WT | 尿素 (Urea) 含量检测试剂盒 (脲酶波氏, 比色法) (2-8℃组分) | U1522046C | 脲酶稀释液 | 无色液体 | 25 mL | 2-8℃. | 每次反应需使用188 μL |
| | | U1522046D | Urea显色液 | 无色液体 | 100 mL | 2-8℃. 避光. | 每次反应需使用1000 μL |
| | | U1522046E | 尿素检测缓冲液 | 无色液体 | 100 mL | 2-8℃. 避光. | 每次反应需使用1000 μL |
| | | U1522046A | 尿素标准 (100mmol/L) | 无色液体 | 1 mL | 2-8℃. | 请按说明配制 |
| U1522046DT | 尿素 (Urea) 含量检测试剂盒 (脲酶波氏, 比色法) (-20℃组分) | U1522046B | 脲酶溶液 | 淡黄色液体 | 0.5 mL | -20℃. 避光. | 每次反应需使用2.1 μL |
1、 无氨蒸馏水
2、 水浴锅或恒温箱、离心管或小试管、分光光度计、比色杯、沸石
1、准备样品：
①血浆、血清：血浆、血清按照常规方法制备，直接用于尿素的测定，-20℃冻存。
②尿液：尿液样品最好处理后测定，方法如下：取 0.6ml 尿液样品，加入沸石 0.3g，加入无氨蒸馏水至 15ml，反复震荡数次，吸附尿液中的游离铵盐，静置后，吸取稀释尿液，所测结果乘以 25，如果尿液比较少，可以等比例减少各试剂的使用量。举例：取 1ml 尿液样品，应加入沸石 0.5g，加入无氨蒸馏水至 25ml，反复震荡数次，吸附尿液中的游离铵盐，静置后，吸取稀释尿液，所测结果乘以 25。
2、配制标准品工作液：取适量的尿素标准 (100mmol/L)，按尿素标准 (100mmol/L)：ddH₂O=1：19 的比例混合，使尿素浓度达到 5mmol/L，即为标准品工作液 - 尿素标准 (5mmol/L)；4℃保存，1 周有效。
3、配制脲酶工作液：取适量的脲酶溶液，按脲酶溶液：脲酶稀释液 = 1：99 的比例混合，即为脲酶工作液；4℃避光保存，1 个月有效。
4、Urea 加样：按照下表设置空白管、标准管、测定管，溶液应按照顺序依次加入，并注意避免产生气泡；如果样品中的 Urea 浓度过高，可以减少样品用量或适当稀释后再进行测定。
5、Urea 测定：充分混匀，37℃水浴 20min，分光光度计检测 560nm 处吸光度，比色杯光径 1.0cm，空白管调零，读取各管吸光度，分别为 A标准、A测定。
尿素 (mmol/L) = (A测定 / A标准) × 5 mmol/L
式中：
A测定 = 测定管的吸光度
A标准 = 标准管的吸光度
成年人血清尿素 | ：2.9~8.2mmol/L |
1、本实验可测定 560 和 630nm 处的吸光度。
2、若无分光光度计，可使用酶标仪检测，需注意加样体积存在差异，检测频次会显著增加。
3、严禁使用铵盐类抗凝剂，易造成检测结果偏高。
4、高浓度氟化物会抑制尿素酶活性，导致结果假性偏低。
5、分光光度计未调零时，空白管 OD 值正常区间 0.08~0.20，5mmol/L 标准管参考区间 0.20~0.50。
6、肉眼观测显色：尿素浓度≤1mmol/L 呈淡绿或淡蓝色；≥2mmol/L 呈蓝色；≥15mmol/L 呈深蓝色，检测数值越靠近上限，结果准确度越高。
7、试剂开封后及时用完，避免影响实验数据。
8、实验操作全程穿戴实验服与一次性手套，做好防护。
6个月有效。低温运输，按要求保存。
Urea, also known as carbamide, is the major nitrogen-containing end product of protein metabolism in mammals and certain fish, and the nitrogen fertilizer with the highest nitrogen content at present. Urea detection methods are generally divided into chemical and enzymatic approaches. The enzymatic method is regarded as an indirect assay: urea is first decomposed into ammonium ions by urease, and ammonium ion yield is then detected based on Berthelot reaction.
The detection principle of Urea Content Assay Kit (Urease-Berthelot, Colorimetric Method): Urease hydrolyzes urea into ammonia and carbon dioxide. Ammonia reacts with phenols under alkaline conditions to form blue indophenol. The production of indophenol is positively correlated with urea content. The absorbance at 560 nm measured by spectrophotometer is used to quantify urea (formerly blood urea nitrogen, BUN) in plasma, serum, urine and other samples from humans and animals. Urine samples are recommended to be pretreated prior to testing. This kit is only for scientific research, not applicable to clinical diagnosis or other purposes.
| Storage Unit No. | Storage Unit Name | Component No. | Component Name | Appearance | 100 reaction | Storage | Quantity Per Test |
| U1522046NT | Urea Content Assay Kit (Urease-Berthelot, Colorimetric Method) (RT Components) | U1522046F | ddH₂O | Colorless liquid | 10 mL | Room temperature | As needed |
| U1522046WT | Urea Content Assay Kit (Urease-Berthelot, Colorimetric Method) (2-8℃ Components) | U1522046C | Urease Diluent | Colorless liquid | 25mL | 2-8℃. | 188 μL for 1 reaction |
| | | U1522046D | Urea Color Developing Solution | Colorless liquid | 100 mL | 2-8℃. Store in the dark. | 1000 μL for 1 reaction |
| | | U1522046E | Urea Assay Buffer | Colorless liquid | 100 mL | 2-8℃. Store in the dark. | 1000 μL for 1 reaction |
| | | U1522046A | Urea Standard (100 mmol/L) | Colorless liquid | 1 mL | 2-8℃. | As needed |
| U1522046DT | Urea Content Assay Kit (Urease-Berthelot, Colorimetric Method) (-20℃ Components) | U1522046B | Urease Solution | Light yellow liquid | 0.5 mL | -20℃. Store in the dark. | 2.1 μL for 1 reaction |
Notes: Each component is individually packaged according to its storage temperature (RT /2‑8 °C / -20 °C) upon shipment, with differentiated temperature‑controlled transportation to guarantee product performance. Orders containing materials requiring multiple temperature zones may be delivered in separate batches; please refer to the actual received goods. Upon receipt, store components separately at the temperatures specified in the component list. The shelf‑life is 6 months from the date of manufacture.
1. Ammonia-free distilled water
2. Water bath/thermostat, centrifuge tubes/test tubes, spectrophotometer, cuvettes, zeolite
1. Sample Preparation
① Plasma & Serum: Prepare samples by conventional methods, ready for detection directly. Store frozen at -20℃.
② Urine: Pretreatment is suggested. Take 0.6mL urine, add 0.3g zeolite, dilute with ammonia-free distilled water to 15mL. Shake repeatedly to adsorb free ammonium salts. Collect supernatant after standing. Multiply the measured value by 25 for final result.Proportional dosage reduction is allowed for limited urine volume. Example: 1mL urine matched with 0.5g zeolite, diluted to 25mL, final result multiplied by 25.
2. Standard Working Solution Preparation
Mix 100mmol/L urea standard solution and double distilled water at 1:19 ratio to prepare 5mmol/L working standard. Store at 4℃, valid for 7 days.
3. Urease Working Solution Preparation
Mix urease solution and urease diluent at 1:99 ratio. Store at 4℃ away from light, valid for 1 month.
4. Sample Loading
Set blank tube, standard tube and test tube as required. Add reagents in sequence and avoid bubble generation. Dilute high-concentration samples appropriately before detection.
5. Colorimetric Detection
Mix thoroughly, incubate in 37℃ water bath for 20 min. Measure absorbance at 560 nm with 1.0cm optical path cuvette. Zero adjustment with blank tube, record absorbance of standard tube and test tube respectively.
Urea content (mmol/L) = (Atest / Astandard) × 5 mmol/L
Atest: Absorbance of test tube
Astandard: Absorbance of standard tube朱传侠
Serum urea for adults: 2.9~8.2mmol/L
Valid for 6 months. Transport under low temperature and store as specified.
| 储运单元货号 | 储运单元名称 | 组分货号 | 组分名称 | 物理外观 | 100 reaction | 储存 | 单次测试使用量 |
| U1522046NT | 尿素 (Urea) 含量检测试剂盒 (脲酶波氏, 比色法) (常温组分) | U1522046F | ddH₂O | 无色液体 | 10 mL | 室温 | 请按说明配制 |
| U1522046WT | 尿素 (Urea) 含量检测试剂盒 (脲酶波氏, 比色法) (2-8℃组分) | U1522046C | 脲酶稀释液 | 无色液体 | 25mL | 2-8℃. | 每次反应需使用188 μL |
| | | U1522046D | Urea显色液 | 无色液体 | 100 mL | 2-8℃. 避光. | 每次反应需使用1000 μL |
| | | U1522046E | 尿素检测缓冲液 | 无色液体 | 100 mL | 2-8℃. 避光. | 每次反应需使用1000 μL |
| | | U1522046A | 尿素标准 (100mmol/L) | 无色液体 | 1 mL | 2-8℃. | 请按说明配制 |
| U1522046DT | 尿素 (Urea) 含量检测试剂盒 (脲酶波氏, 比色法) (-20℃组分) | U1522046B | 脲酶溶液 | 淡黄色液体 | 0.5 mL | -20℃. 避光. | 每次反应需使用2.1 μL |
注：发货时各组分依据储存温度 (RT/2‑8℃/-20℃) 独立包装，差异化温控运输以保障产品性能。同一订单含多温区物料可能分批次送达，请以实际收货为准。收货后按组分表标注温度分类存放，有效期自生产之日起 6 个月。
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 J2613216 |
分析证书 | U1522046 |
| I2623561 |
分析证书 | U1522046 |
| I2623562 |
分析证书 | U1522046 |
| I2618982 |
分析证书 | U1522046 |
| I2612047 |
分析证书 | U1522046 |
| ZJ26F0636637 |
分析证书 | U1522046 |
| H2617505 |
分析证书 | U1522046 |
| G2628435 |
分析证书 | U1522046 |
| G2613369 |
分析证书 | U1522046 |
| F2624404 |
分析证书 | U1522046 |
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