EnzymoPure™ 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
UBE1负责泛素-蛋白质异肽键形成的第一步。UBE1通过形成高能硫酯键来催化遍在蛋白C端羧基的活化。然后，该活化的遍在蛋白通过E2 / E3偶联级联转移到靶蛋白的赖氨酸上。引发体外反应的关键成分。
This enzyme is responsible for the first step in ubiquitin-protein isopeptide bond formation, by forming a high-energy thioester bond with ubiquitin. The ubiquitin is activated by first adenylating with ATP its Cterminal glycine residue (Gly76) and thereafter linking this residue to the side chains of a cysteine residue in UBE1, yielding an ubiquitin-UBE1 thiolester and free AMP and PPi. The activated ubiquitin is then transferred to a lysine of the targeted protein via the E2-E3 conjugation cascade. UBE1 is a critical component for the initiation of any in vitro conjugation reactions. We recommend an initial Recombinant Human Ubiquitin Activating Enzyme (UBE1) concentration of 50-200 nM.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 H2429377 |
分析证书 | U388907 |
危险品化学品经营许可证（带存储）