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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
木聚糖(Xylan)是植物细胞壁的主要组成成分，它也是可利用的丰富的半纤维素。木聚糖酶(Xylanase)指能专一降解半纤维素、木聚糖为低聚木糖和木糖的一组酶的总称。木聚糖酶主要来源于细菌和真菌，包括厌氧菌、需氧菌、嗜温微生物、嗜热微生物和极端微生物等。 木聚糖酶主要包括3类：①β-1,4-D-内切木聚糖酶(EC3.2.1.8)，从木聚糖主链的内部切割β-1，4糖苷键，使木聚糖溶液的黏度迅速降低；②β-1,4-D-外切木聚糖酶(EC3.2.1.92)，以单个木糖为切割单位，作用于木聚糖的非还原性末端，使反应体系还原性不断增加；③β-木糖苷酶(EC3.2.1.37)，切割低聚木糖，水解产物主要为木糖、木二糖及木三糖等低聚木糖。 根据酶的理化特性，木聚糖酶又可分为2大类：即分子量小于30ku的碱性木聚糖酶和分子量大于30ku的酸性木聚糖酶。通常细菌可以同时产生酸、碱性木聚糖酶，但真菌通常只产生低分子量的碱性木聚糖酶。从本质上讲，木聚糖酶都具有外切酶和内切酶活性，只是活性高低不同而已。作为一种工业用酶制剂，木聚糖酶具有潜在的应用价值。首先木聚糖酶可以将农业和木材工业的废物中的木聚糖转化为木糖；其次，Wong等建议木聚糖酶可应用在果汁澄清、咖啡、植物油和淀粉提取加工过程中。 该试剂盒检测原理是：木聚糖酶能将木聚糖降解成还原性寡糖和单糖，在沸水浴条件下可以与３,５-二硝基水杨酸(DNS)试剂发生显色反应。反应液颜色的深度与酶解产生的还原糖量成正比，而还原糖的生成量又与反应液中木聚糖酶的活力成正比。因此，通过测定反应液颜色的强度，可以计算反应液中木聚糖酶的活力。本产品仅用于科研领域，不用于临床诊断或其他用途。
Xylan is a major component of plant cell walls and is also an abundant and utilizable hemicellulose. Xylanase refers to a group of enzymes that specifically degrade hemicellulose and xylan into xylooligosaccharides and xylose. Xylanases are mainly derived from bacteria and fungi, including anaerobic bacteria, aerobic bacteria, mesophilic microorganisms, thermophilic microorganisms, and extremophiles. Xylanases are mainly classified into three types: ① β-1,4-D-endoxylanase (EC 3.2.1.8), which cleaves β-1,4 glycosidic bonds from within the main chain of xylan, rapidly reducing the viscosity of the xylan solution; ② β-1,4-D-exoxylanase (EC 3.2.1.92), which acts on the non-reducing ends of xylan, cleaving individual xylose units and continuously increasing the reducing power of the reaction system; and ③ β-xylosidase (EC 3.2.1.37), which cleaves xylooligosaccharides, yielding mainly xylose, xylobiose, xylotriose, and other xylooligosaccharides as hydrolysis products. Based on their physicochemical properties, xylanases can also be divided into two main categories: alkaline xylanases with a molecular weight of less than 30 kDa and acidic xylanases with a molecular weight greater than 30 kDa. Generally, bacteria can produce both acid and alkaline xylanases simultaneously, while fungi typically only produce low-molecular-weight alkaline xylanases. Essentially, all xylanases possess both exo-enzyme and endo-enzyme activities, differing only in their relative activity levels. As an industrial enzyme preparation, xylanase has significant potential applications. Firstly, xylanase can convert xylan from agricultural and wood industry waste into xylose. Secondly, researchers such as Wong have suggested that xylanase can be used in processes like juice clarification, and in the extraction and processing of coffee, vegetable oils, and starch. The detection principle of this kit is as follows: Xylanase degrades xylan into reducing oligosaccharides and monosaccharides. Under boiling water bath conditions, these products can react with 3,5-dinitrosalicylic acid (DNS) reagent to produce a color reaction. The intensity of the reaction solution's color is directly proportional to the amount of reducing sugars produced by the enzymatic hydrolysis, which in turn is directly proportional to the xylanase activity in the reaction solution. Therefore, by measuring the color intensity of the reaction solution, the xylanase activity can be calculated. This product is intended for research purposes only and is not suitable for clinical diagnosis or other uses.
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