This product contains 4% SDS, 20% glycerol, 0.02% phenol red and 0.125 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.
Note: Please use after bringing back to normal temperature.

Explanation：

Preparation of non-reducing sample
A protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.
Preparation of reducing sample (reducing with 2-Mercaptoethanol)
Add 10 μL of 2-Mercaptoethanol per 90 μL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE.

规格

英文名称

2X SDS-PAGE Sample Buffer Phenol Red (2-Mercaptoethanol free) [for Electrophoresis]

储存条件

室温

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分子类型

生物试剂/缓冲液

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