JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Han Zhang,Lulu Wang,Xiaolin Yao,Zonghan Wang,Leina Dou,Lihong Su,Man Zhao,Jing Sun,Daohong Zhang,Jianlong Wang
An innovative lateral flow competition immunoassay (LFCIA) for detecting clenbuterol (CL) was developed by employing the advantages of the coomassie brilliant blue (CBB) staining method. An antibody stained by CBB was used both as a recognition reagent and as a chromogenic probe, enabling the simple but sensitive LFCIA of CL. The CBB-based LFCIA exhibited sensitivity for CL with a detection limit of 2 ng mL–1. Furthermore, this strategy was preliminarily verified by screening for CL in milk, pork tenderloin, and swine liver with recoveries ranging from 81 to 102%. Compared with conventional LFCIAs, the use of CBB as a signal label not only avoided the complicated material synthesis and surface modification process but also simplified the cross-linking with antibodies, meanwhile reducing the steric hindrance and increasing the possibility of immune recognition reactions, which was propitious for the effective utilization of antibodies. Taking advantages of the simplicity, rapidity, and cost-effectiveness, the CBB-based LFCIA may have potential for on-demand monitoring of general harmful small molecules by changing the kind of the staining antibody.
研究领域:
分析化学
·
食品安全
·
生物技术
✓ 已验证
DOI
PubMed
高影响因子
Journal of Zhejiang University-SCIENCE B
·
2016
·
IF 1.303
Sovichea Lay,Hai-ning Yu,Bao-xiang Hu,Sheng-rong Shen
A pre-treatment methodology for clenbuterol hydrochloride (CLEN) isolation and enrichment in a complex matrix environment was developed through exploiting molecularly imprinted polymers (MIPs). CLEN-imprinted polymers were synthesized by the combined use of ally-β-cyclodextrin (ally-β-CD) and methacrylic acid (MAA), allyl-β-CD and acrylonitrile (AN), and allyl-β-CD and methyl methacrylate (MMA) as the binary functional monomers. MAA-linked allyl-β-CD MIPs (M-MAA) were characterized by Fourier transform-infrared (FT-IR) spectroscopy and a scanning electron microscope (SEM). Based upon the results, M-MAA polymers generally proved to be an excellent selective extraction compared to its references: AN-linked allyl-β-CD MIPs (M-AN) and MMA-linked allyl-β-CD MIPs (M-MMA). M-MAA polymers were eventually chosen to run through a molecularly imprinted solid-phase extraction (MISPE) micro-column to enrich CLEN residues spiked in pig livers. A high recovery was achieved, ranging from 91.03% to 96.76% with relative standard deviation (RSD) ≤4.45%.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Yiwei Tang,Jingwen Gao,Xiuying Liu,Xue Gao,Tao Ma,Xiaonan Lu,Jianrong Li
As an ideal biomimetic antibody, molecularly imprinted polymer (MIP) has shown great promise in immunoassays. Here, we developed a covalent imprinting method to prepare MIP artificial antibody towards clenbuterol on the well surface of a microtiter plate. Template molecule (clenbuterol) was splited by hydrolysis with functional monomer and removed by methanol/acetic acid solution, and then three-dimensional cavities were fabricated in the MIP, which can rebind template molecule via hydrogen bond interaction. Using this artificial antibody, we developed a novel biomimetic enzyme-linked immunosorbent assay (ELISA) with excellent sensitivity and specificity. The linear range for clenbuterol was 10 −5 to 1000 μg L −1 , with a detection limit of 10 −7 μg L −1 . Cross reactivity of this MIP artificial antibody to other four structural analogs was less than 0.93%. This method was applied to determine clenbuterol in real samples with satisfactory result, suggesting a promising application of the biomimetic ELISA in food and environmental analysis.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 12
·1-10
·
IF 5.705
Ge Yu,Camarada María Belén,Xu Lanjiao,Qu Mingren,Liang Huan,Zhao Erlong,Li Mingfang,Wen Yangping
A nanocomposite was prepared from graphene-like two-dimensional black phosphorene (BP, an allotrope of phosphorus) and nafion (Nf) treated with isopropanol (IP). A glassy carbon electrode (GCE) modified with this nanocomposite was found to be a viable sensor for voltammetric determination of clenbuterol (CLB). Unlike previously reported pure BP, the BP nanocomposite was stable towards water and oxygen. Its morphology, structure, electrochemically active surface area and electrochemical stability were investigated. The BP-Nf (IP) modified GCE displayed good electrochemical stability and electrocatalytic capacity with a low working potential of 0.94 V (vs. SCE), excellent peak current response for CLB in a linear concentration range of 0.06–24 μM with a detection limit of 3.7 nM (3σ/m) and a sensitivity of 0.14 μA·μM −1 ·cm −2 under optimal conditions. A sensing mechanism for the electro-oxidation of CLB was suggested and verified by density functional theory calculations under imitation of aqueous solution conditions. The sensor was successfully applied to the determination of CLB in bovine meat and bovine serum samples.
Highly-stable black phosphorene (BP) nanocomposite based on Nafion (Nf) was used to modify a glassy carbon electrode (GCE). It is shonw to be a viable electrochemical platform for sensitive voltammetric determination of trace clenbuterol (CLB) in bovine beef and bovine serum.
研究领域:
分析化学
·
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2016
·Vol. 183
·期 3
·1089-1097
·
IF 4.831
Zhang Zhihong,Duan Fenghe,He Linghao,Peng Donglai,Yan Fufeng,Wang Minghua,Zong Wei,Jia Chunxiao
A nanocomposite consisting of zinc sulfide quantum dots and polyaniline (ZnSQD@PANI) was placed on a gold electrode along with antibody against clenbuterol to give an amperometric immunosensor for clenbuterol. Compared to the use of pristine PANI, the electrode modified with the ZnSQD@PANI nanocomposite adsorbs clenbuterol antibody much better and therefore exhibits higher sensitivity to clenbuterol. The biosensor, when operated at a working potential of 0.21 V (vs. Ag/AgCl), displays a detection limit as low as 5.5 pg⋅mL −1 and works over the 0.01 to 10 ng⋅mL −1 concentration range. Related species such as salbutamol and ractopamine, urine components such as urea and uric acid, and the ions Ca(II), Na(I), and K(I) do not interfere.
A nanocomposite of zinc sulfide quantum dots and polyaniline was synthesized and utilized as the electrode material for detecting clenbuterol hydrochloride (CH). Sensing includes immunoglobulin G anchoring, adsorption of clenbuterol antibody, and detection of clenbuterol.
研究领域:
分析化学
·
生物传感技术
·
纳米材料科学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Sujun Liu,Kai Shi
The residue of clenbuterol (CLB) in food poses a potential harm to human health. Herein, we presented an electrochemical aptasensor ( E -A-CRISPR) based on employing an aptamer as a specific recognition element and asymmetric CRISPR-Cas12a as signal amplifiers for sensitive, and selective detection of CLB. In this E -A-CRISPR system, the target CLB bound to the aptamer and initiated cascade signal amplification through the DNase activity of CRISPR-Cas12a with two competitive gRNAs. Upon amplification, the active Cas12a cleaved the methylene blue-labeled hairpin probe on the electrode, reducing the peak current. Under optimal conditions, the E -A-CRISPR system showed a wide linear range (1 pM-100 nM) and a low detection limit (500 fM). This system could detect CLB in potable water, pig liver, and pork samples, showing significant potential for food safety monitoring. To our knowledge, this study is the first to use a CRISPR-Cas12a powered system for electrochemical sensing of CLB.
研究领域:
食品安全
·
生物传感
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Yiwei Tang,Ziyuan Gao,Shuo Wang,Xue Gao,Jingwen Gao,Yong Ma,Xiuying Liu,Jianrong Li
A novel fluorescence probe based on upconversion particles, YF 3 :Yb 3+ , Er 3+ , coating with molecularly imprinted polymers (MIPs@UCPs) has been synthesized for selective recognition of the analyte clenbuterol (CLB), which was characterized by scan electron microscope and X-ray powder diffraction. The fluorescence of the MIPs@UCPs probe is quenched specifically by CLB, and the effect is much stronger than the NIPs@UCPs (non-imprinting polymers, NIPs). Good linear correlation was obtained for CLB over the concentration range of 5.0–100.0 μg L −1 with a detection limit of 0.12 μg L −1 ( S/N =3). The developed method was also used in the determination of CLB in water and pork samples, and the recoveries ranged from 81.66% to 102.46% were obtained with relative standard deviation of 2.96–4.98% ( n =3). The present study provides a new and general tactics to synthesize MIPs@UCPs fluorescence probe with highly selective recognition ability to the CLB and is desirable for application widely in the near future.
研究领域:
分析化学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Lu Yang,Tong Zhai,Jing Wang,Hong Zheng,Jing-Min Liu,Shuo Wang
To achieve high throughput detection of clenbuterol while reducing matrix interference, we developed a simple and integrated nano-inspection platform. Herein, based on the simplicity and convenience of paper chip, a multi-layer paper chip-chemiluminescence (CL) sensor was designed in the form of “48-well plate” type. The all-in-one paper chip-CL sensor has integrated the covalent organic frameworks (COFs) layer for sample matrix purification, high affinity nanobody layer for specific capture of target, and CL rapid response layer for the final determination. The signal readout was realized by the inhibitory effect of clenbuterol on the K 3 [Fe(CN) 6 ] CL system. Detection of clenbuterol in pork meat was verified by measuring the CL intensity and satisfactory recoveries (85.9 %–97.3 %) were achieved. 48 samples could be simultaneously detected on one-chip in one-test, favoring the high-throughput detection. Especially, the work explored a new pathway for design of portable analytical instruments to achieve immediate on-site detection of hazards.
研究领域:
分析化学
·
食品安全检测
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 2
·513-520
·
IF 1.938
Yun Xie,Huafang Chang,Kang Zhao,Jianguo Li,Hong Yang,Liyun Mei,Shouming Xu,Anping Deng
In this study, we present a novel immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) for the sensitive and quantitative determination of clenbuterol in urine samples. The principle of this new ICA is similar to that based on colloidal gold particles, but using AuMBA@Ag–Ab [e.g. the polyclonal antibody of clenbuterol labelled Au@Ag core–shell nanoparticles (NPs) sandwiched with a Raman reporter (4-mercaptobenzoic acid, MBA)] as a probe. The clenbuterol in solution and the clenbuterol–ovalbumin conjugate previously coated on the test line of the ICA strip competed for limited antibody binding sites on the probe. The specific Raman scattering intensity of MBA on the test line was measured for the quantitative detection of clenbuterol. This proposed assay was completed within 15 min. The IC50 and limit of detection (LOD) values of the assay for clenbuterol were found to be 0.02 ng mL−1 and 0.24 pg mL−1, respectively. The relative standard deviations (RSD) of the signal obtained from 10 points along the middle part of the test line were within 4.3–8.7%. There was no cross-reactivity (CR) of the assay with ractopamine or phenylethanolamine A, and only 19% CR with salbutamol. The recoveries of clenbuterol from spiked swine urine samples were in the range of 93.8–112.4% with RSD values in range of 5.2–11.5% (n = 9). The proposed SERS-based ICA was demonstrated to be a rapid, simple and ultrasensitive analytical method for detecting clenbuterol in urine samples and could also be applied for the determination of other target analytes in different matrices.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
ELECTROANALYSIS
·
2018
·Vol. 30
·期 11
·2744-2749
·
IF 2.851
Xin Xiao,YiHui Wang,Wei Tan,HongJie Liu,YangYang Duan,XiaoBo Zhang,DongEn Zhang,YouXiang Jiang,Jie Wang,JunYan Gong,JuanJuan Ma,Tao Yang,ZhiWei Tong
A novel multilayer-shaped CeO 2 with obvious electrocatalytic activity was synthesized using a simple heat treatment method. The structure and morphology of the multilayer-shaped CeO 2 were characterized by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. Clenbuterol, a kind of β-agonist drugs, has recently become a serious issue to human body due to the illegal addition in the food additive for livestocks. The drug residue in the human body may cause muscular pain, cardiac palpitation, glaucoma, prostatitis, dizziness and and even death in old people. Therefore, the detection of clenbuterol (CLB) has a important significance. The as-prepared CeO 2 was found to have unique electrocatalytic oxidation activities towards clenbuterol (CLB). The electrochemical sensor was constructed by fixing stable nafion-modified multilayer-shaped CeO 2 film on a glassy carbon electrode. Compared with the CeO 2 -modified electrode and the nafion-modified electrode, the nafion-modified multilayer-shaped CeO 2 electrode showed better electrocatalytic performance for clenbuterol in HClO 4 solution. There was a good linear relationship between the oxidation peak current and the concentration of CLB between 0.39∼2.79 μmol/L, and the detection limit was 0.08 μmol/L (R=0.99). The nafion-modified multilayer-shaped CeO 2 electrode exhibited high stability and reproducibility.
研究领域:
分析化学
·
纳米材料科学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Li Liu,Chenghong Long,Shenghui Wei,Yu Sun
The determination of clenbuterol is crucial for food safety and sport science. In this work, we use tetraethyl orthosilicate and (3-aminopropyl)triethoxysilane as cross-linking agents and functional monomers for the electrodeposition of SiO 2 films with the addition of clenbuterol. After the elution process, the prepared electrode can be used for the sensitive determination of clenbuterol due to the molecular imprinting effect. Under optimum conditions, results show that the current of clenbuterol decreases linearly in the range of 2 μM to 0.1 mM, and the detection limit is 31 nM. The proposed electrochemical sensor also exhibits excellent stability and reproducibility, showing its high potential for use in sensing clenbuterol in food samples.
研究领域:
食品安全
·
分析化学
·
运动科学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Yingnan Liu,Yaqing Xiao,Min Yu,Yuanyuan Cao,Fan Li,Pei Jia,Du Guo,Xinyu Sun,Li Wang
In view of the potential harm caused by illegal feeding of clenbuterol (CLB) in the livestock industry, herein, a novel ratiometric fluorescent probe based on graphene quantum dots (GQDs)@[Ru(bpy)3]2+ was elaborately constructed for CLB detection. In this probe, GQDs acted as response signals, and their fluorescence was remarkably quenched by CLB through the diazotization-coupling reaction. As for [Ru(bpy)3]2+ as a reference signal, its fluorescence was hardly affected. The intensity ratio of two fluorophores showed good linearity with CLB concentration in the range of 0.05–40 μM, accompanied by visualization of fluorescence variation from yellow to red. The detection limit was as low as 0.029 μM. Particularly, the probe was successfully used to detect CLB in pork and beef samples with satisfactory recoveries. To our knowledge, this is the first report on a ratiometric fluorescent probe for the detection of CLB, which possesses broad application prospects in food safety risk monitoring.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）