产品简介
7-AAD（7-氨基放线菌素D）能够嵌入双链DNA，选择性结合GC区域的荧光染料。7-AAD不能透过活细胞的细胞膜，但能穿透死细胞或濒死细胞的细胞膜，因此可替代碘化丙啶（PI）用于细胞坏死及凋亡的检测评估。
7-AAD可在488 nm氩激光下激发，具有较大的斯托克斯位移（Stokes shift），其最大发射波长为647 nm。基于这些光谱特性，7-AAD可与荧光素异硫氰酸酯（FITC）、藻红蛋白（PE）等其他488 nm激发的荧光素联合使用。
7-AAD活性染色液为即用型试剂，适用于流式细胞术单参数或多参数分析中细胞样本的活性评估。
使用说明
该试剂已预稀释至推荐使用浓度。建议每个样本100μl（含1×10^6个细胞），每个样本中加入2.5-5 μl 7-AAD细胞活性染色液，室温避光温育10分钟后进行检测分析。
注意事项
1. 如果有细菌或真菌污染，可能会严重影响检测效果。
2. 染色后宜尽快检测，时间过长可能会导致坏死细胞的数量增加。
3. 细胞经固定、通透和RNase处理后也可以使用7-AAD检测细胞周期，但更常用的是碘化丙啶(PI)。
4. 荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
5. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Description
7-AAD (7-amino-actinomycin D) is a fluorescent dye that intercalates into double-stranded DNA (GC rich regions). It is excluded from viable cells, but can penetrate cell membranes of dead or dying cells. Therefore, it can be used instead of propidium iodide (PI) for the evaluation of cell death and apoptosis.
7-AAD can be excited at 488 nm with an argon laser. It has a relatively large Stokes shift, emitting at a maximum wavelength of 647 nm. Because of these spectral characteristics, 7-AAD can be used in combination with other fluorochromes excited at 488 nm such as fluorescein isothiocyanate (FITC) and phycoerythrin (PE).
7-AAD Viability Staining Solution is a ready-to-use reagent suitable for the evaluation of cell viability in mono- or multiparametric analyses of flow cytometry.
Instruction for use
This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). It is recommended to use 7-AAD Viability Staining Solution at 2.5-5 μl per test and incubate for 10 minutes in the dark at room temperature before analysis.
Precautions
1. Bacterial or fungal contamination may severely compromise assay performance.
2. Samples should be analyzed promptly after staining, as prolonged incubation may increase necrotic cell populations.
3. 7-AAD can be utilized for cell cycle analysis following fixation, permeabilization, and RNase treatment, though propidium iodide (PI) remains more routinely employed for this application.
4. All fluorochromes undergo photobleaching; minimize light exposure to retard fluorescence decay.
5. Wear protecting clothing, gloves to ensure personal safety and health protection.
ZJ25F0927762
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