脂滴黄色探针（聚集诱导发光）是一种基于AIE原理开发的三苯胺类衍生物，具有典型的AIE特性。产品具有细胞跨膜能力，只需简单地和细胞孵育，即可通过被动转运完成跨膜运输。
产品特性
脂滴黄色探针（聚集诱导发光）具有良好的聚集诱导发光特性，可以特异性标记多种细胞的脂滴结构，在与脂滴结合前后由于聚集状态的变化，其荧光强度会产生极为明显的变化，而未与脂滴结合的荧光探针基本不会发射出荧光信号。
图 1. HeLa细胞上的激光共聚焦成像效果图
与常见染料不同，
脂滴黄色探针（聚集诱导发光）的斯托克斯位移大，可以明显与其他染料进行区分，减少成像中串色的可能性。同时
脂滴黄色探针（聚集诱导发光）的生物相容性好，成像浓度高，可在多次扫描的状态仍然保证稳定的荧光信号输出，非常适合多次成像。
产品性质
化学式（Formula）
| C₂₈H₁₉NO₂
|
分子量（Molecular Weight）
| 401.47 g/mol
|
纯度（Purity）
| ≥98% (HPLC)
|
工作浓度（Working Concentration）
| 5-10 µM
|
半峰宽（Full width at half maximum）
| 575 nm-675 nm
|
最大吸收/发射波长（nm）
| λabs =485 nm / Em= 630 nm
|
产品优势
1、细胞毒性相对较小，可以进行活细胞成像；
2、强抗光漂白能力，40 次共 15 分钟的激光扫描，发射荧光强度不变；
3、背景信号低，快速成像。
实验方法
1、染料储备液准备：短暂离心，吹打均匀，将探针溶液适当分装后避光保存于-20℃或更低温度；
2、染料工作液准备：取1 µL
脂滴黄色探针（聚集诱导发光）储备液加入到1-2 mL 细胞培养液或适当的缓冲液中(例如 PBS)，得到终浓度5-10 μM 的
脂滴黄色探针（聚集诱导发光）工作液；
3、细胞染色。适量的工作染液孵育贴壁细胞 30 min（最好置于细胞培养箱中），PBS洗涤3次，用共聚 焦荧光显微镜或荧光显微镜观察，激发波长设为488 nm；收集550-650 nm的信号。
注意事项
1、使用前请先短暂离心；
2、第一次使用时请先配置好母液后分装，于-20℃保存，避免反复冻融；
3、为了您的安全和健康，请穿实验服并戴一次性手套操作；
4、本品仅适用于科研用途。
Lipid droplets Yellow Probe (AIE)
is a triphenylamine derivative developed based on the AIE principle and has typical AIE characteristics. The product has cell transmembrane ability; simply by incubating with cells, transmembrane transport can be completed through passive transport.Product Characteristics
Lipid droplets Yellow Probe (AIE) has excellent aggregation-induced emission properties and can specifically label the lipid droplet structures in various cells. Due to changes in the aggregation state before and after binding to lipid droplets, its fluorescence intensity undergoes extremely significant changes. The fluorescent probes that do not bind to lipid droplets basically do not emit fluorescent signals.
Figure 1. Laser confocal imaging effect diagram of HeLa cells
Different from common dyes,
Lipid droplets Yellow Probe (AIE)
has a large Stokes shift, which can be clearly distinguished from other dyes, reducing the possibility of crosstalk in imaging. Meanwhile,
Lipid droplets Yellow Probe (AIE)
has good biocompatibility and high imaging concentration, and can ensure stable fluorescence signal output even in the state of multiple scans, making it very suitable for multiple imaging.
Product Properties
| Product Properties | Details |
|---|
| Formula | C₂₈H₁₉NO₂
|
| Molecular Weight | 401.47 g/mol
|
| Purity | ≥98% (HPLC) |
| Working Concentration | 5-10 µM
|
| Full Width at Half Maximum | 575 nm-675 nm
|
| Max Absorption/Emission Wavelength (nm) | λabs =485 nm / Em= 630 nm |
Product Advantages
1.Relatively low cytotoxicity, suitable for live cell imaging;
2.Strong anti - photobleaching ability; after 40 laser scans totaling 15 minutes, the emitted fluorescence intensity remains unchanged;
3.Low background signal, enabling rapid imaging.
Experimental Methods
1.Preparation of dye stock solution: Centrifuge briefly, pipette and mix evenly. After appropriately aliquoting the probe solution, store it in the dark at -20 °C or a lower temperature;
2.Preparation of dye working solution: Take 1 µL of the AIE Lipid Droplet Yellow Probe stock solution and add it to 1 - 2 mL of cell culture medium or an appropriate buffer (such as PBS) to obtain an AIE Lipid Droplet Yellow Probe working solution with a final concentration of 5 - 10 μM;
3.Cell staining. Incubate adherent cells with an appropriate amount of the working dye solution for 30 min (preferably placed in a cell incubator). Wash 3 times with PBS. Observe using a confocal fluorescence microscope or a fluorescence microscope. Set the excitation wavelength to 488 nm; collect signals at 550 - 650 nm.
Notes
1.Please centrifuge briefly before use;
2.When using for the first time, prepare the mother solution first, then aliquot it, and store at -20 °C to avoid repeated freezing and thawing;
3.For your safety and health, please wear a lab coat and disposable gloves during operation;
4.This product is only for scientific research use.
ZJ25F1028444
危险品化学品经营许可证（带存储）