溶酶体红色探针（聚集诱导发光）是一种基于AIE原理开发的三苯胺类衍生物，具有典型的AIE特性。产品具有细胞跨膜能 力，只需简单地和细胞孵育，即可通过被动转运完成跨膜运输。
产品特性
溶酶体红色探针（聚集诱导发光）具有良好的聚集诱导发光特性，可以特异性标记多种细胞的溶酶体结构，在与溶酶体结合前后由 于聚集状态的变化，其荧光强度会产生极为明显的变化，而未与溶酶体结合的荧光探针基本不会发射出荧光信号。
图 1. HeLa细胞上的激光共聚焦成像效果图
与常见染料不同，
溶酶体红色探针（聚集诱导发光）斯托克斯位移大，可以明显与其他染料进行区分，减少成像中串色的可能性。 同时
溶酶体红色探针（聚集诱导发光） 的生物相容性好，成像浓度高，可在多次扫描的状态仍然保证稳定的荧光信号输出，非常适合多 次成像。
产品性质
化学式（Formula）
| C₄₀H₄₄N₂O₅
|
分子量（Molecular Weight）
| 659 g/mol
|
纯度（Purity）
| ≥98% (HPLC)
|
工作浓度（Working Concentration）
| 1-20 μM
|
半峰宽（Full width at half maximum）
| 600 nm-700 nm
|
最大吸收/发射波长（nm）
| λabs = 490 nm / Em = 700 nm |
产品优势
1、细胞毒性相对较小，可以进行活细胞成像；
2、强抗光漂白能力，40 次共 15 分钟的激光扫描，发射荧光强度不变；
3、背景信号低，快速成像。
实验方法
1、染料储备液准备：短暂离心后，向一管
溶酶体红色探针（聚集诱导发光）中加入400 µL无水DMSO，超声处理并吹 打均匀后，得到浓度为2 mM 的
溶酶体红色探针（聚集诱导发光）储备液，适当分装后避光保存于-20℃或更低温度；
2、染料工作液准备：取5 µL
溶酶体红色探针（聚集诱导发光）储备液加入到1-2 mL 细胞培养液或适当的缓冲液中(例如 PBS)，得到终浓度5-10 μM 的
溶酶体红色探针（聚集诱导发光）工作液，可根据实验需求调整终浓度；
3、细胞染色。适量的工作染液孵育贴壁细胞15-30 min（最好置于细胞培养箱中），PBS清洗三次，用 共聚焦荧光显微镜或荧光显微镜观察，激发波长设为488 nm，收集640-750 nm的信号。
注意事项
1、第一次使用前请先短暂离心，配置好母液后分装，于-20℃保存，避免反复冻融；
2、本品出现黑色沉淀为正常现象，每次使用前请超声；
3、为了您的安全和健康，请穿实验服并戴一次性手套操作；
4、本品仅适用于科研用途。
Lysosome Red Probe (AIE)
is a triphenylamine derivative developed based on the AIE principle and has typical AIE characteristics. The product has the ability to cross cell membranes; simply by incubating it with cells, trans-membrane transport can be completed through passive transport.
Product Characteristics
Lysosome Red Probe (AIE) has excellent aggregation-induced emission properties and can specifically label the lysosome structures of various cells. Due to changes in the aggregation state before and after binding to lysosomes, its fluorescence intensity undergoes extremely significant changes. The fluorescent probes that do not bind to lysosomes basically do not emit fluorescent signals.
Figure 1. Laser confocal imaging effect diagram of HeLa cells
Different from common dyes,
Lysosome Red Probe (AIE) has a large Stokes shift, which can be clearly distinguished from other dyes, reducing the possibility of crosstalk in imaging. Meanwhile,
Lysosome Red Probe (AIE) has good biocompatibility and a high imaging concentration, and can ensure stable fluorescence signal output even in the state of multiple scans, making it very suitable for multiple imaging.
Product Properties
| Formula | C₄₀H₄₄N₂O₅
|
| Molecular Weight | 659 g/mol
|
| Purity | ≥98% (HPLC) |
| Working Concentration | 1-20 μM
|
| Full Width at Half Maximum | 600 nm-700 nm
|
| Max Absorption/Emission Wavelength (nm) | λabs = 490 nm / Em = 700 nm |
Product Advantages
1.Relatively low cytotoxicity, suitable for live cell imaging;
2.Strong anti-photobleaching ability; the fluorescence intensity remains unchanged after 40 laser scans totaling 15 minutes;
3.Low background signal, enabling rapid imaging.
Experimental Methods
1.Preparation of dye stock solution: After a brief centrifugation, add 400 µL of anhydrous DMSO to a tube of
Lysosome Red Probe (AIE). After ultrasonic treatment and pipetting to mix evenly, a 2 mM
Lysosome Red Probe (AIE) stock solution is obtained. Appropriately aliquot it and store it protected from light at -20 °C or a lower temperature;
2.Preparation of dye working solution: Take 5 µL of the
Lysosome Red Probe (AIE) stock solution and add it to 1-2 mL of cell culture medium or an appropriate buffer (such as PBS) to obtain an
Lysosome Red Probe (AIE) working solution with a final concentration of 5-10 μM. The final concentration can be adjusted according to experimental needs;
3.Cell staining: Incubate adherent cells with an appropriate amount of the working dye solution for 15-30 minutes (preferably in a cell incubator). Wash three times with PBS. Observe using a confocal fluorescence microscope or a fluorescence microscope. Set the excitation wavelength to 488 nm and collect signals at 640-750 nm.
Precautions
1.Before the first use, please centrifuge briefly. After preparing the mother solution, aliquot it and store it at -20 °C to avoid repeated freezing and thawing;
2.The appearance of black precipitates in this product is a normal phenomenon. Please sonicate before each use;
3.For your safety and health, please wear a lab coat and disposable gloves during operation;
4.This product is only for scientific research purposes.
ZJ25F1028445
危险品化学品经营许可证（带存储）