BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存,避光,室温,-20°C储存,避免反复冻融。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
葡萄糖（Glucose, Dextrose, Glu）又称玉米葡糖，简称葡糖，化学式为 C₆H₁₂O₆，分子量 180.16，是自然界分布最广、最为重要的单糖，属于多羟基醛。酶学方法是生化检测中测定葡萄糖的常用手段，其中以葡萄糖氧化酶法和己糖激酶法最为常用，这类方法具有灵敏度、准确度与精密度均较高、反应条件温和、对葡萄糖具有专一性且不受其他糖及还原物质干扰、操作简便、适用于自动分析仪等特点。
葡萄糖检测试剂盒(GOD-POD微板法) 又称葡萄糖氧化酶法或葡萄糖氧化酶‑过氧化物酶偶联法，其检测原理为：在葡萄糖氧化酶的催化下，葡萄糖被氧化成葡萄糖酸，同时消耗溶液中的氧并产生过氧化氢，过氧化氢与显色底物反应生成红色的醌类化合物，反应初期过氧化氢的生成量与葡萄糖浓度成正比，可通过酶标仪在 505 nm 处进行检测。
该试剂盒可用于人或动物血清、血浆、脑脊液、细胞、组织等样本中葡萄糖含量的定量测定，但不宜直接用于尿液中葡萄糖的检测，其中 Glu 标准液浓度为 5 mmol/L（相当于 90 mg/dl）。本试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
临用前，按酚试剂：酶试剂=1：1混匀，即GOD-POD工作液，4℃保存。 |
自备仪器和试剂
操作步骤（仅供参考）
1. 样本处理
1.1 血清、血浆、脑脊液样品
从待测样品中分离出的血清或血浆不应有溶血，直接测定，如超过线性范围 (30 mmol/L)，用生理盐水或PBS稀释后测定。
1.2 细胞样品
(1) 取适量的细胞（一般推荐>10⁶以上），1000g离心10 min，弃上清，留取沉淀。
(2) 用PBS或生理盐水清洗1~2次，1000g离心10 min，弃上清，留取沉淀。
(3) 加入200~300 μL的PBS或生理盐水匀浆，冰浴条件下超声破碎细胞，功率300W，每次3~5 s，间隔30 s，重复3~5次，亦可手动匀浆，制备好的匀浆液不可离心；亦可用1~2% Triton X-100冰浴30~60 min，制备好的裂解液不可离心。
1.3 组织样品
准确称取适量组织样品，按质量（g）：生理盐水或PBS（ml）=1：9的比例，加入生理盐水或PBS，冰浴条件下手动或机械匀浆，2500~3000g离心10 min，取上清。
2. 加样
取96孔板，参考下表设置空白孔、标准孔、测定孔，依次加入各种溶液，充分混匀，37℃水浴锅或45℃恒温箱中孵育15 min。
|
3. 测定
冷却后，测定505 nm处吸光度，以空白孔调零，读取标准孔、测定孔的吸光度，分别记为A标准、A测定。
4. 计算
Glu (mmol/L)=A测定/A标准×5
Glu (mg/L)=A测定/A标准×900
参考区间：
健康成年人空腹葡萄糖：3.9~6.1 mmol/L (70~110 mg/dl)
备注：Glu标准 (5 mmol/L)=90 mg/dl=900 mg/L
性能指标
|
注意事项
1. 配制好的GOD-POD工作液，4℃避光保存，1周有效，低温试剂避免反复冻融，以免失效或效率下降。
2. 测定标本用血清或以草酸钾-氟化钠抗凝的血浆（可抑制葡萄糖的分解），可直接用于检测脑脊液中的葡萄糖含量，待测样品如不能及时测定应置于2~8℃保存，3天内稳定。
3. 尿葡萄糖目前多采用此法进行定量测定，但不能直接检测，需先用班氏法对尿液样品做半定量试验，按测出的大概含量，用蒸馏水稀释尿液使葡萄糖含量在3 mg/mL以下，再进行检测，计算结果乘以稀释倍数即可；因为未经处理的尿液中尿酸等还原性物质的浓度较高，影响过氧化物酶反应，可能会造成结果假性偏低。
4. 随着时间的延长，低浓度样品亦会显红色，所以15 min后应及时检测，时间不宜过久。
5. 采用酶标仪未调零情况下空白参考范围在0.04~0.09之间，5 mmol/L标准参考范围在0.25~0.45之间，由于仪器设备、操作方法等不同，参考范围会有差异。
6. 该试剂盒测定下限为0.1 mmol/L，测定上限为30 mmol/L；以肉眼观察，浓度≤0.6 mmol/L几乎呈无色，浓度在0.7 mmol/L即可显淡红色，浓度≥2.5 mmol/L可显红色，一般情况下接近上限比接近下限更准确。
7. 本法线性范围可达30 mmol/L，如果样品葡萄糖浓度过高，结果可能呈假性降低，应用生理盐水或PBS等稀释后重测，结果乘以稀释倍数。
8. 试剂开封后请尽快使用，以防影响后续实验效果。
9. 为了您的安全和健康，请穿实验服并戴一次性手套操作。
Glucose (Glucose, Dextrose, Glu), also known as corn sugar, is the most widely distributed and most important monosaccharide in nature. It is a polyhydroxy aldehyde with the chemical formula C₆H₁₂O₆ and a molecular weight of 180.16. Enzymatic methods are commonly used for glucose determination in biochemical assays, with the glucose oxidase method and hexokinase method being the most frequently used. These methods are characterized by high sensitivity, accuracy, and precision; mild reaction conditions; specificity for glucose without interference from other sugars or reducing substances; simple operation; and suitability for automated analyzers.
The Glucose Assay Kit (GOD-POD Microplate Method), also known as the Glucose Oxidase Method or Glucose Oxidase-Peroxidase Coupling Method, operates on the following principle: Under the catalysis of glucose oxidase, glucose is oxidized to gluconic acid, consuming oxygen from the solution and producing hydrogen peroxide. The hydrogen peroxide then reacts with a chromogenic substrate to form a red quinone compound. In the initial reaction phase, the amount of hydrogen peroxide produced is proportional to the glucose concentration, which can be detected using a microplate reader at 505 nm.
This kit can be used for the quantitative determination of glucose content in samples such as human or animal serum, plasma, cerebrospinal fluid, cells, and tissues. However, it is not suitable for direct detection of glucose in urine. The concentration of the Glu Standard provided is 5 mmol/L (equivalent to 90 mg/dL). This kit is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Before use, mix the Phenol Reagent and Enzyme Reagent at a 1:1 ratio to prepare the GOD-POD Working Solution. Store at 4°C.
Reagents, consumables and Equipments not provided
Microplate reader
Centrifuge tubes or 96-well plates, homogenizer, centrifuge, water bath or incubator
Physiological saline or PBS
Procedure (For Reference Only)
1. Sample Preparation
1.1 Serum, Plasma, Cerebrospinal Fluid Samples
Separate serum or plasma from the test sample should be non-hemolyzed. Measure directly. If the concentration exceeds the linear range (30 mmol/L), dilute with physiological saline or PBS before measurement.
1.2 Cell Samples
(1) Take an appropriate number of cells (generally recommended > 10⁶ cells). Centrifuge at 1000 g for 10 minutes. Discard the supernatant, retaining the pellet.
(2) Wash 1-2 times with PBS or physiological saline. Centrifuge at 1000 g for 10 minutes. Discard the supernatant, retaining the pellet.
(3) Add 200–300 μL of PBS or physiological saline for homogenization. Disrupt cells by sonication in an ice bath (300 W power, 3-5 seconds each time with 30-second intervals, repeated 3-5 times). Manual homogenization can also be used. The prepared homogenate should not be centrifuged. Alternatively, add 1-2% Triton X-100 and incubate in an ice bath for 30–60 minutes. The prepared lysate should not be centrifuged.
1.3 Tissue Samples
Accurately weigh an appropriate amount of tissue sample. Add physiological saline or PBS at a ratio of sample weight (g): saline/PBS (mL) = 1:9. Homogenize manually or mechanically in an ice bath. Centrifuge at 2500–3000 g for 10 minutes and collect the supernatant.
2. Sample Addition
Take a 96-well plate and set up Blank, Standard, and Assay wells according to the table below. Add the various solutions sequentially, mix thoroughly, and incubate in a 37°C water bath or 45°C incubator for 15 minutes.
|
3. Measurement
After cooling, measure the absorbance at 505 nm. Zero the reader with the Blank well. Read the absorbance of the Standard and Assay wells, recorded as A<sub>Standard</sub> and A<sub>Assay</sub>, respectively.
4. Calculation
Glu (mmol/L) = Glu (mmol/L) = AAssay / AStandard × 5
Glu (mg/L) = AAssay / AStandard × 900
Reference Interval:
Healthy adults, fasting blood glucose: 3.9–6.1 mmol/L (70–110 mg/dL)
Note: Glu Standard (5mmol/L) = 90 mg/dL = 900 mg/L
Performance Characteristics
|
Notes
The prepared GOD-POD Working Solution should be stored at 4°C protected from light and is stable for 1 week. Avoid repeated freeze-thaw cycles of refrigerated reagents to prevent inactivation or reduced efficiency.
Use serum or plasma anticoagulated with potassium oxalate-sodium fluoride (which inhibits glucose breakdown) for measurement. This method can be directly used to detect glucose content in cerebrospinal fluid. If the test samples cannot be measured promptly, store them at 2-8°C; they are stable for up to 3 days.
Although this method is often used for quantitative determination of urine glucose, it cannot be measured directly. First, perform a semi-quantitative test on the urine sample using the Benedict's method. Based on the approximate content obtained, dilute the urine sample with distilled water to bring the glucose concentration below 3 mg/mL. Then perform the assay, multiplying the result by the dilution factor. Untreated urine contains high concentrations of reducing substances like uric acid that can interfere with the peroxidase reaction, potentially causing falsely low results.
Low-concentration samples may also turn red over extended periods. Therefore, measure promptly after the 15-minute incubation; avoid prolonged delays.
If the microplate reader is not zeroed, the blank reference range is typically between 0.04 and 0.09, and the 5 mmol/L standard reference range is typically between 0.25 and 0.45. Reference ranges may vary depending on factors such as instrument and operating method.
The detection limit of this kit is 0.1 mmol/L, and the upper limit is 30 mmol/L. Visually, concentrations ≤ 0.6 mmol/L appear almost colorless, concentrations around 0.7 mmol/L show a faint red color, and concentrations ≥ 2.5 mmol/L show a distinct red color. Generally, results near the upper limit are more accurate than those near the lower limit.
The linear range of this method reaches up to 30 mmol/L. If the sample glucose concentration is too high, results may be falsely low. Dilute the sample with physiological saline or PBS and re-assay, multiplying the result by the dilution factor.
Use the reagents as soon as possible after opening to avoid affecting subsequent experimental results.
For your safety and health, please wear a lab coat and disposable gloves during operation.
| A1371397 | 组分 | 200 T | 500 T | 储存 |
| A1371397A | 酚试剂 | 25 mL | 65 mL | 室温，避光储存。 |
| A1371397B | 酶试剂 | 25 mL | 65 mL | -20℃，避免反复冻融，避光储存 |
| A1371397C | Glu标准 (5mmol/L) | 1 mL | 1 mL | 2-8℃. |
| A1371397D | ddH₂O | 1 mL | 1 mL | 室温 |
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 J2623502 |
分析证书 | A1371397 |
| J2617014 |
分析证书 | A1371397 |
| J2610170 |
分析证书 | A1371397 |
| J2609465 |
分析证书 | A1371397 |
| I2622339 |
分析证书 | A1371397 |
| ZJ26F0939123 |
分析证书 | A1371397 |
| ZJ26F0939122 |
分析证书 | A1371397 |
| I2604225 |
分析证书 | A1371397 |
| G2615281 |
分析证书 | A1371397 |
| G2608555 |
分析证书 | A1371397 |
| F2624399 |
分析证书 | A1371397 |
| F2611341 |
分析证书 | A1371397 |
| ZJ25F0724703 |
分析证书 | A1371397 |
| ZJ25F0724704 |
分析证书 | A1371397 |
| ZJ25F0724705 |
分析证书 | A1371397 |
| ZJ25F0724706 |
分析证书 | A1371397 |
| D2617620 |
分析证书 | A1371397 |
| D2610581 |
分析证书 | A1371397 |
| C2625698 |
分析证书 | A1371397 |
| ZJ26F0333277 |
分析证书 | A1371397 |
| K2507197 |
分析证书 | A1371397 |
危险品化学品经营许可证（带存储）