计算溶液所需的质量、体积或浓度。
碱性乙醇分化液
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
碱性乙醇分化液由乙醇、氢氧化钾、去离子水等混合配制而成，是改良Highman刚果红染色液的主要成分之一，主要用于刚果红染色淀粉样物质的分化。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
1、10%中性福尔马林、蒸馏水、系列乙醇、二甲苯或环保脱蜡液、中性树胶
2、甲醇刚果红染色液、Mayer苏木素染色液
操作步骤(仅供参考)：
1、常规固定，常采用10%的中性福尔马林固定液，常规脱水包埋。
2、切片厚度4μm，常规二甲苯或环保脱蜡液脱蜡至水。
3、放入甲醇刚果红染色液(0.5%)浸染10-30min，弃余液。
4、碱性乙醇分化液分化1-5s，立即入水终止分化，水洗2次后镜下控制至恰当程度。
5、自来水冲洗5min。
6、用Mayer苏木素染色液浅染细胞核1-2min或更短时间。自来水冲洗10min。
7、逐级常规乙醇脱水，二甲苯或环保透明液透明，中性树胶封固。
染色结果：
在光学显微镜下，淀粉样物质呈红色，细胞核呈蓝色；
在偏光显微镜下，淀粉样物质呈黄绿色的双折光。
改良Highman染色液(淡染效果，蓝色为核)
注意事项：
1、切片脱蜡应尽量干净，否则影响染色效果。
2、甲醇刚果红染色液染色时尽量采用浸染，如果滴染，应置于湿盒防止溶液挥发。
3、碱性乙醇分化液分化步骤很重要，分化时间较短，胶原纤维也被染成红色；分化过度，淀粉样物质也被脱色；如果脱色过度，可以将切片清洗后重新用刚果红染色液浸染。
4、脱水应迅速，避免脱色。
5、本产品由氢氧化钾、乙醇等组成，有轻微腐蚀性。请小心操作。
6、由于组织特异性或环境变化等因素，有时会出现红色不明显的情况，应增加染色时间。
7、为了您的安全和健康，请穿实验服并戴一次性手套操作。
8、试剂开封后请尽快使用，以防影响后续实验效果。
Alkaline Ethanol Differentiation Solution is prepared by mixing ethanol, potassium hydroxide, deionized water, and other components. As one of the main ingredients of the modified Highman Congo Red Staining Solution, it is primarily used for the differentiation of amyloid substances in Congo Red staining. This reagent is for research use only and not intended for clinical diagnosis or other purposes.
Materials to Be Prepared by Users
1. 10% neutral formalin, distilled water, a series of ethanol solutions, xylene or environment-friendly dewaxing solution, neutral balsam
2. Methanol Congo Red Staining Solution, Mayer's Hematoxylin Staining Solution
Operating Procedures (for Reference Only)
1. Perform routine fixation (10% neutral formalin fixative is commonly used), followed by routine dehydration and embedding.
2. Prepare sections with a thickness of 4 μm, then conduct routine dewaxing to water using xylene or environment-friendly dewaxing solution.
3. Immerse the sections in 0.5% Methanol Congo Red Staining Solution for 10-30 minutes, then discard the residual solution.
4. Differentiate the sections with Alkaline Ethanol Differentiation Solution for 1-5 seconds. Immediately transfer the sections to water to terminate differentiation, rinse twice with water, and then check under a microscope to ensure the differentiation reaches an appropriate level.
5. Rinse the sections with tap water for 5 minutes.
6. Stain the cell nuclei lightly with Mayer's Hematoxylin Staining Solution for 1-2 minutes or a shorter period. Rinse with tap water for 10 minutes.
7. Perform routine gradient dehydration with ethanol, clear the sections with xylene or environment-friendly clearing solution, and then mount the sections with neutral balsam.
Staining Results
Under an optical microscope: Amyloid substances appear red, and cell nuclei appear blue.
Under a polarizing microscope: Amyloid substances exhibit yellow-green birefringence.
Modified Highman Staining (light staining effect; blue structures are nuclei)
Precautions
1. Ensure sections are fully dewaxed; otherwise, the staining effect will be affected.
2. When staining with Methanol Congo Red Staining Solution, immersion staining is recommended as much as possible. If drop staining is adopted, place the sections in a humid chamber to prevent the solution from evaporating.
3. The differentiation step with Alkaline Ethanol Differentiation Solution is crucial: If the differentiation time is too short, collagen fibers will also be stained red. If over-differentiation occurs, amyloid substances will be decolorized. In case of over-decolorization, rinse the sections, then re-immerse them in Congo Red Staining Solution for staining.
4. Dehydration should be carried out quickly to avoid decolorization.
5. This product contains potassium hydroxide, ethanol, and other components, and has slight corrosiveness. Handle with care.
6. Due to factors such as tissue specificity or environmental changes, the red color may sometimes be indistinct. In such cases, extend the staining time.
7. For your safety and health, wear a lab coat and disposable gloves during operation.
8. After opening the reagent, use it as soon as possible to avoid affecting the results of subsequent experiments.
规格
规格或纯度
BioReagent,生物染色剂,用于显微镜,1×
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Alkaline Ethanol Differentiation Solution
储存条件
避光,室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F1230639 |
分析证书 | A1507825 |
技术文档和文章
溶液计算器
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