计算溶液所需的质量、体积或浓度。
酸性乙醇分化液 (0.3%)
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
分化作用是指组织染色后用某些特定的溶液将组织过多结合的染色剂脱去，这个过程称为分化作用，所用的溶液称为分化液。在HE染色和其他染色中常用1%盐酸乙醇作为分化液，因酸能破坏苏木素的醌型结构，使组织与色素分离而退色，再进行伊红染色，才能保证细胞核与细胞浆染色的分明。
酸性乙醇分化液(0.3%)主要由0.3%稀酸、乙醇、去离子水等组成，是一种非常重要的辅助试剂。本产品可用于HE染色、Masson三色染色苏木素染色后的分化，分化时间较慢，约需30秒，便于更精细地控制分化时间，特别适合对于苏木素染色分化的要求非常精细或者对于分化操作不是很熟练的实验人员。经本产品处理后，核质着色更加清晰分明。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
操作步骤(仅供参考)：
1、根据实验具体要求操作。
2、一般分化20-40s，立即用水或蓝化液终止。
注意事项：
1、密闭保存，一旦开启尽快用完，因为其有效成分易挥发。
2、为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、 试剂开封后请尽快使用，以防影响后续实验效果。
Differentiation refers to the process of removing excess stain that has bound to tissues using specific solutions after tissue staining; the solution used for this purpose is called a differentiation solution. In HE staining (Hematoxylin-Eosin staining) and other staining procedures, 1% hydrochloric acid-ethanol is commonly used as a differentiation solution. This is because the acid can destroy the quinone structure of hematoxylin, causing the separation of tissues and pigments (thus leading to decolorization). Only after this step is eosin staining performed can a clear distinction between the staining of cell nuclei and cytoplasm be ensured.
The Acidic Ethanol Differentiation Solution (0.3%) is mainly composed of 0.3% dilute acid, ethanol, deionized water, etc., and is a very important auxiliary reagent. This product can be used for differentiation after hematoxylin staining in HE staining and Masson trichrome staining. It has a relatively slow differentiation rate, taking approximately 30 seconds, which allows for more precise control of the differentiation time. It is particularly suitable for researchers who have extremely high precision requirements for hematoxylin staining differentiation or are not very proficient in differentiation operations. After treatment with this product, the staining of cell nuclei and cytoplasm becomes clearer and more distinct. This reagent is for scientific research use only and is not suitable for clinical diagnosis or other purposes.
Operating Procedures (for reference only)
1. Operate in accordance with the specific requirements of the experiment.
2. Generally, the differentiation process takes 20-40 seconds, and should be immediately terminated by rinsing with water or using a bluing solution.
Precautions
1. Store in a sealed container. Once opened, use it as soon as possible, as its active ingredients are volatile.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. After opening the reagent, use it promptly to avoid affecting the results of subsequent experiments.
规格
别名
酸性乙醇分化液
英文别名
Acidic Ethanol Differentiation Solution
规格或纯度
BioReagent, 生物染色剂, 用于显微镜, 0.3%
稳定性与储存
Store at room temperature long term (24 months). Store in the dark.
英文名称
Acidic Ethanol Differentiation Solution (0.3%)
储存条件
避光,室温
运输条件
常规运输
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
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