D2503510计算溶液所需的质量、体积或浓度。
为什么选择此级别
提供 ≥95% 纯度，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
-20°C储存,充氩,干燥。超低温运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
N-Hydroxysulfosuccinimide (NHS) esters of biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.
Varieties of biotin NHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein.
Application：
Sulfo-NHS-LC-LC-biotin finds extensive applications in scientific research for the purpose of protein labeling and detection. Its utilization in protein purification allows for the isolation of biotinylated proteins from intricate mixtures. Moreover, it plays a vital role in protein-protein interaction studies, enabling investigations into the binding affinity and specificity of proteins. Additionally, it serves as a valuable tool in protein quantification, facilitating the measurement of specific protein levels within a given sample.
Features and benefits:
• Protein labeling—biotinylate antibodies to facilitate immobilization, purification or detection using streptavidin resins or probes
• Cell surface labeling—biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes
• Amine-reactive—reacts with primary amines (-NH2), such as the side-chain of lysines (K) or the amino-termini of polypeptides
• Soluble—charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds
• Irreversible—forms permanent amide bonds; spacer arm cannot be cleaved
• Doubly long —spacer arm (total length added to target) is 22.4 angstroms; this extended arm helps to minimize steric hindrance for biotin binding
规格
别名
Aladdin™ 磺化 NHS-LC-LC-生物素
规格或纯度
≥95%
英文名称
Aladdin™ Sulfo NHS-LC-LC-Biotin
储存条件
-20°C储存,充氩,干燥
运输条件
超低温运输
纯度
≥95%
名称和识别符
PubChem SID
分子类型
未知
INCHI
1S/C26H41N5O10S2.Na/c32-20(27-14-8-2-4-12-23(35)41-31-22(34)15-19(25(31)36)43(38,39)40)10-3-1-7-13-28-21(33)11-6-5-9-18-24-17(16-42-18)29-26(37)30-24;/h17-19,24H,1-16H2,(H,27,32)(H,28,33)(H2,29,30,37)(H,38,39,40);/t17-,18-,19?,24-;/m0./s1
InChi Key
RWNPTJCDHAXLGI-BJPAGVOZSA-N
Smiles
C1C(C(=O)N(C1=O)OC(=O)CCCCCNC(=O)CCCCCNC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)O.[Na]
Isomeric SMILES
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCCCC(=O)ON3C(=O)CC(C3=O)S(=O)(=O)O)NC(=O)N2.[Na]
PubChem CID
分子量
669.74
技术文档
高级数据
三维结构
交互式化学结构模型
化学和物理性质
溶解性
DMF (Dimethylformamide), DMSO (Dimethylsulfoxide), Water
敏感性
对空气敏感；对热敏感
质检证书（CoA,COO,BSE/TSE 和分析图谱)
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
Copyright © 2023至今 上海阿拉丁生化科技股份有限公司 保留所有权利。
危险品化学品经营许可证（带存储）