Mouse anti Human Adiponectin Antibody, Monoclonal (H2), could be used for WB, IHC and so on.
Application:
WB: 0.01-2µg/mL IHC: 5-30µg/mL
Protein Function
Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Adiponectin Mouse mAb (Ab087545) - Western Blot
All lanes: Adiponectin Mouse mAb (Ab087545) at 1/2000 dilution
Samples: Lysates at 5 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 26 kDa
Observed band size: 28 kDa
Exposure time: 50.0 s
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Human Cardiac Muscle Tissue
Primary Ab: 20 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Adiponectin Mouse mAb (Ab087545) - IHC
DAB staining on IHC
Sample: Rat Cardiac Muscle Tissue
Primary Ab: 30 µg/mL Adiponectin Mouse mAb (Ab087545)
Control: Used PBS instead of primary antibody
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
5-30 µg/mL
0.01-2 µg/mL
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1011774 |
分析证书 | Ab087545 |
| ZJ24F1011854 |
分析证书 | Ab087545 |
| ZJ23F1201912 |
分析证书 | Ab087545 |
| ZJ23F1201913 |
分析证书 | Ab087545 |
| ZJ23F1201914 |
分析证书 | Ab087545 |
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