ALDH6A1 Mouse mAb (Ab088099) - Western Blot All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 57 kDa Observed band size: 54 kDa Exposure time: 28.1 s
ALDH6A1 Mouse mAb (Ab088099) - Western Blot All lanes: ALDH6A1 Mouse mAb (Ab088099) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 57 kDa Observed band size: 54 kDa Exposure time: 96.3 s
ALDH6A1 Mouse mAb (Ab088099) - IF/ICC Immunofluorescent analysis of ALDH6A1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ALDH6A1 Mouse mAb (Ab088099) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
ALDH6A1 Mouse mAb (Ab088099) - IHC Immunohistochemical analysis of ALDH6A1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with ALDH6A1 Mouse mAb (Ab088099) at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
ZJ24F0809641
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