ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Mouse anti Human CDA Antibody, Monoclonal (K1E006_15C8), could be used for WB, IHC, IP and so on.
CDA Mouse mAb (Ab095561) - Western Blot
All lanes: CDA Mouse mAb (Ab095561) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 15.0 s
CDA Mouse mAb (Ab095561) - IHC
IHC-P was performed using sections of the formalin-fixed paraffin-embedded spleen tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with CDA Mouse mAb (Ab095561) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in red pulp are positively stained.
CDA Mouse mAb (Ab095561) - IP
Immunoprecipitation was performed by incubation of 2.5 µg CDA Mouse mAb (Ab095561) with SK-OV-3 lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. CDA Mouse mAb (Ab095561) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Lane 1: SK-OV-3 lysate
Lane 2: CDA immunoprecipitated from SK-OV-3 lysate by CDA Mouse mAb (Ab095561)
Lane 3: The same as Lane 2 but Mouse IgG was used as IgG isotype control antibody
Result: CDA Mouse mAb (Ab095561) can immunoprecipitate CDA.
1/1000
1/100
1/400
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F1114177 |
分析证书 | Ab095561 |
| ZJ24F1114176 |
分析证书 | Ab095561 |
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