Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Acts as a component of the TACC3/ch-TOG/clathrin complex proposed to contribute to stabilization of kinetochore fibers of the mitotic spindle by acting as inter-microtubule bridge.
宿主种属
小鼠(Mouse)
特异性
Clathrin light chain/CLTA
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant full length protein of human Clathrin light chain/CLTA.
阳性对照
WB: K562, HeLa, Hep G2, HEK293, NIH/3T3 and C6 cell lysates. IHC: Human kidney, lymph node tissues.
Clathrin light chain/CLTA Mouse mAb (Ab096687) - Western Blot All lanes: Clathrin light chain/CLTA Mouse mAb (Ab096687) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 24 kDa Observed band size: 32, 36 kDa Clathrin light chain/CLTA Mouse mAb (Ab096687) recognizes different isoforms of Clathrin light chain/CLTA Exposure time: 118.9 s
Clathrin light chain/CLTA Mouse mAb (Ab096687) - IHC Immunohistochemical staining of paraffinembedded Human Kidney tissue within the normal limits using Clathrin light chain/CLTA Mouse mAb (Ab096687). Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min (1/500).
Clathrin light chain/CLTA Mouse mAb (Ab096687) - IHC Immunohistochemical staining of paraffinembedded Human lymph node tissue within the normal limits using Clathrin light chain/CLTA Mouse mAb (Ab096687). Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min (1/500).