CYC antibody | CYC_HUMAN antibody | CYCS antibody | Cytochrome c antibody | Cytochrome c somatic antibody | HCS antibody | THC4 antibody | Monoclonal Antibody to Cytochrome C
规格或纯度
无载体, ExactAb™, 无叠氮钠, 已验证, 高性能, 见COA
生化机理
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
WB: HEK293, Jurkat, HeLa, Hep G2, A549 and LNCaP cell lysates. IHC: Human cerebellum, cardiac muscle, small intestine, lymph node tissues. ICC/IF: Hep G2, HeLa cells.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
C2
Format
Whole IgG
亚型
Mouse IgG2a
轻链亚型
kappa
纯化方法
Protein A + Protein G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.05% Proclin 300, pH7.4
防腐剂
0.05% Proclin 300
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
短期储存2-8℃(1-2周);长期储存-20℃(24个月);收货后建议分装,避免反复冻融
图片
Cytochrome C Mouse mAb (Ab098695) - Western Blot All lanes: Cytochrome C Mouse mAb (Ab098695) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 12 kDa Observed band size: 16 kDa Exposure time: 7.9 s
Cytochrome C Mouse mAb (Ab098695) - IF/ICC FITC staining on IF Sample: Human Hep G2 cell Primary Ab: 20 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 5 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IF/ICC FITC staining on IF Sample: Human HeLa cell Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC DAB staining on IHC Sample: Human Cerebellum Tissue Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC DAB staining on IHC Sample: Human Cardiac Muscle Tissue Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC DAB staining on IHC Sample: Human Small intestine Tissue Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Cytochrome C Mouse mAb (Ab098695) - IHC DAB staining on IHC Sample: Human Lymph node Tissue Primary Ab: 30 μg/mL Cytochrome C Mouse mAb (Ab098695) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.