Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.
In the nucleus is involved in transcriptional regulation. Associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. Implicated in transcriptional activation of CDKN1A; the function involves STAT3 and SRC. Involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Post-translational: Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues (PubMed:27134172). Signaling via SEMA4C promotes phosphorylation at Tyr-1248 (PubMed:17554007). Dephosphorylated by PTPN12 (PubMed:27134172).
宿主种属
小鼠(Mouse)
特异性
ErbB2/Her2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant protein of human ERBB2 (AA 676-1255).
阳性对照
WB: SK-OV-3 cell lysate. IHC: Human breast tissue. IF/ICC: COS7 cells. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY ERBB2.
ErbB2/Her2 Mouse mAb (Ab102062) - Western Blot All lanes: ErbB2/Her2 Mouse mAb (Ab102062) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 138 kDa Observed band size: 179 kDa Exposure time: 100.0 s
ErbB2/Her2 Mouse mAb (Ab102062) - IHC Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using ErbB2/Her2 Mouse mAb (Ab102062). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120 °C for 3 min.
ErbB2/Her2 Mouse mAb (Ab102062) - IF/ICC ErbB2/Her2 Mouse mAb (Ab102062) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ERBB2.
ErbB2/Her2 Mouse mAb (Ab102062) - Flow HEK293T cells transfected with either pCMV6-ENTRY ERBB2 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by ErbB2/Her2 Mouse mAb (Ab102062), and then analyzed by flow cytometry.
ZJ25F0623355
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