ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Mouse anti Human EXOSC10/RRP6 Antibody, Monoclonal (K29029_1D2), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/200
IP: 1/100
Protein Function:
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.
EXOSC10/RRP6 Mouse mAb (Ab102491) - Western Blot
All lanes: EXOSC10/RRP6 Mouse mAb (Ab102491) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/40000 dilution
Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 150.0 s
EXOSC10/RRP6 Mouse mAb (Ab102491) - IHC
IHC-P analysis of skin tissue by EXOSC10/RRP6 Mouse mAb (Ab102491).
IHC-P was performed using sections of the formalin-fixed paraffin-embedded skin tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with EXOSC10/RRP6 Mouse mAb (Ab102491) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in epidermis are positively stained at the nuclei.
EXOSC10/RRP6 Mouse mAb (Ab102491) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of EXOSC10/RRP6 Mouse mAb (Ab102491) with HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. EXOSC10 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Lane 1: HEK293 lysate
Lane 2: EXOSC10 immunoprecipitated from HEK293 lysate by EXOSC10/RRP6 Mouse mAb (Ab102491)
Lane 3: The isotype control antibody instead of EXOSC10/RRP6 Mouse mAb (Ab102491) in HEK293 lysate
Result: EXOSC10/RRP6 Mouse mAb (Ab102491) can immunoprecipitate EXOSC10.
1/1000
1/100
1/200
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0707808 |
分析证书 | Ab102491 |
| ZJ24F0707807 |
分析证书 | Ab102491 |
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