Mouse anti Human FER Antibody, Monoclonal (1457CT181.12.17), could be used for WB, IHC and so on. Application: WB: 1/1000 IHC: 1/100-1/500 Protein Function: Tyrosine kinase of the non-receptor type. Probably performs an important function, perhaps in regulatory processes such as cell cycle control.
规格
产品名称
FER Mouse mAb
别名
酪氨酸激酶受体C单克隆抗体 | FER抗体 | FER 小鼠单克隆抗体 | FER 小鼠单抗
英文别名
PPP1R74 | EC 2.7.10 | EC 2.7.10.2 | fer (fps/fes related) tyrosine kinase | Fer | p94-Fer | Phosphoprotein NCP94 | Proto-oncogene c-Fer | TYK3 | TYK3phosphoprotein NCP94 | Tyrosine kinase 3 | tyrosine-protein kinase Fer | c FER antibody | Feline encephali
规格或纯度
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
宿主种属
小鼠(Mouse)
特异性
FER
种属反应性
人(Human),小鼠(Mouse)
免疫原
Recombinant FER expressed in E.coli (AA 1-300).
阳性对照
WB: MCF7, A549, Hep G2 and NIH/3T3 cell lysates. IHC: Human liver tissue.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
1457CT181.12.17
Format
Whole IgG
亚型
Mouse IgG1
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 0.09% Sodium azide, pH 7.4
防腐剂
0.09% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4°C 运输。送达后,等分并储存于 -20°C。避免冷冻/解冻循环。
图片
FER Mouse mAb (Ab103180) - Western Blot All lanes: FER Mouse mAb (Ab103180) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 94 kDa Observed band size: 93 kDa Exposure time: 168.2 s
FER Mouse mAb (Ab103180) - Western Blot All lanes: FER Mouse mAb (Ab103180) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 94 kDa Observed band size: 93 kDa Exposure time: 30.0 s
FER Mouse mAb (Ab103180) - IHC FER Mouse mAb (Ab103180) staining FER in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.