Mouse anti Human Ferritin Light Chain Antibody, Monoclonal (H1), could be used for WB, IHC and so on. Application WB:0.5-2 μg/mL IHC: 5-20 µg/mL Protein Function Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.
Recombinant Ferritin Light Chain expressed in E.coli (AA 2-175)
阳性对照
WB: K562, THP-1, A549, C6 and NIH/3T3 cell lysates. IHC: Human Spleen tissue, Human Liver tissue.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
H1
Format
Whole IgG
亚型
Mouse IgG1
轻链亚型
kappa
纯化方法
Protein A + Protein G purified
浓度
见COA
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 50% Glycerol, 0.02% Sodium azide, pH7.4
防腐剂
0.02% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
图片
Ferritin Light Chain Mouse mAb (Ab103206) - Western Blot All lanes: Ferritin Light Chain Mouse mAb (Ab103206) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 224.0 s
Ferritin Light Chain Mouse mAb (Ab103206) - Western Blot All lanes: Ferritin Light Chain Mouse mAb (Ab103206) at 1/500 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 20 kDa Exposure time: 370.7 s
Ferritin Light Chain Mouse mAb (Ab103206) - IHC DAB staining on IHC Sample: Human Spleen Tissue Primary Ab: 20 µg/mL Ferritin Light Chain Mouse mAb (Ab103206) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.
Ferritin Light Chain Mouse mAb (Ab103206) - IHC DAB staining on IHC Sample: Human Liver Tissue Primary Ab: 20 µg/mL Ferritin Light Chain Mouse mAb (Ab103206) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.