Mouse anti Human Hck Antibody, Monoclonal (1508CT602.13.1), could be used for WB, ICC, IF and so on. Application WB: 1/2000-1/4000 ICC/IF: 1/25 Protein Function May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
ExactAb™, Validated, Carrier Free, High performance, 0.5 mg/mL
宿主种属
小鼠(Mouse)
特异性
Hck
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse)
免疫原
Recombinant human HCK expressed in E.coli (AA 1-526).
阳性对照
WB: THP-1 and Hep G2 cell lysates. ICC/IF: Hep G2 cells.
偶联
Unconjugated
克隆类型
单克隆抗体
克隆号
1508CT602.13.1
Format
Whole IgG
亚型
Mouse IgG1
轻链亚型
kappa
纯化方法
Protein G purified
浓度
0.5 mg/mL
分子类型
抗体
产品线
生物产品
储存与运输
物理外观
Liquid
储存缓冲液
10mM PBS, 0.09% Sodium azide, pH7.4
防腐剂
0.09% Sodium azide
储存条件
-20°C储存,避免反复冻融
运输条件
超低温运输
稳定性与储存
4℃ 短期储存（1-2 周）。-20℃长期保存（24 个月）。收货后建议分装。避免冷冻/解冻循环。
图片
Hck Mouse mAb (Ab106982) - Western Blot All lanes: Hck Mouse mAb (Ab106982) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa Exposure time: 10.0 s
Hck Mouse mAb (Ab106982) - Western Blot All lanes: Hck Mouse mAb (Ab106982) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 60 kDa Observed band size: 58 kDa Exposure time: 60.0 s
Hck Mouse mAb (Ab106982) - IF/ICC Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hep G2 (human liver hepatocellular carcinoma cell line) cells labeling HCK with Hck Mouse mAb (Ab106982) at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
ZJ24F0303024
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