Mouse anti Human Kindlin 2 Antibody, Monoclonal (OTI14A11), could be used for WB, IHC, IF/ICC, IP and so on.
规格
产品名称
Kindlin 2 Mouse mAb
别名
Kindlin 2单克隆抗体 | 丝裂原诱导蛋白2抗体
英文别名
FLJ34213 | FLJ44462 | UNC112 | FERM2_HUMAN | Fermitin family homolog 2 | Fermt2 | KIND2 | Kindlin-2 | MIG-2 | MIG2 | Mitogen-inducible gene 2 protein | PH domain-containing family C member 1 | Pleckstrin homology domain containing family C (with FERM doma
规格或纯度
敲除验证, ExactAb™, 已验证, 见COA
生化机理
Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.
宿主种属
小鼠(Mouse)
特异性
FERMT2
种属反应性
人(Human),大鼠(Rat),小鼠(Mouse),狗(Dog),猴(Monkey)
免疫原
Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector
阳性对照
WB: Hep G2, HeLa, A549, NIH/3T3 and C6 cell lysates. IHC: Human pancreas tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY FERMT2, Hep G2 cells. IP: HEK293T lysates.
Kindlin 2 Mouse mAb (Ab112146) - Western Blot All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/2000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution
Predicted band size: 78 kDa Observed band size: 72 kDa Exposure time: 100.0 s
Kindlin 2 Mouse mAb (Ab112146) - Western Blot All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 78 kDa Observed band size: 72 kDa Exposure time: 30.0 s
Kindlin 2 Mouse mAb (Ab112146) - KO Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT) and FERMT2-Knockout HeLa cells (KO) were separated by SDSPAGE and immunoblotted with Kindlin 2 Mouse mAb (Ab112146) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.
Kindlin 2 Mouse mAb (Ab112146) - IF/ICC Kindlin 2 Mouse mAb (Ab112146) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2.
Kindlin 2 Mouse mAb (Ab112146) - IF/ICC Immunofluorescent staining of Hep G2 cells using Kindlin 2 Mouse mAb (Ab112146).
Kindlin 2 Mouse mAb (Ab112146) - IHC Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using Kindlin 2 Mouse mAb (Ab112146). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min).
ZJ25F0117170
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