Mouse anti Human LAT2 Antibody, Monoclonal (K52022_7A12), could be used for WB, IHC and so on. Application: WB: 1/1000 IHC: 1/100-1/200 Protein Function Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2. Post-translational Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2. May be polyubiquitinated.
规格
产品名称
LAT2 Mouse mAb
别名
B淋巴细胞链接激活蛋白抗体 | LAT2小鼠单克隆抗体 | LAT2小鼠单抗
英文别名
LAB antibody | LAT2 antibody | Linker for activation of B cells antibody | Linker for activation of B-cells antibody | Linker for activation of T cells family member 2 antibody | Linker for activation of T cells transmembrane adaptor 2 antibody | Linker f
规格或纯度
ExactAb™, Validated, Carrier Free, 1.0 mg/mL
宿主种属
小鼠(Mouse)
特异性
LAT2
种属反应性
人(Human)
免疫原
Recombinant protein of human LAT2 (AA 121-220).
阳性对照
WB: U937, Raji and THP-1 cell lysates. IHC: Human tonsil tissue.
LAT2 Mouse mAb (Ab112832) - Western Blot All lanes: LAT2 Mouse mAb (Ab112832) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 27 kDa Exposure time: 62.8 s
LAT2 Mouse mAb (Ab112832) - IHC IHC analysis of tonsil tissue by LAT2 Mouse mAb (Ab112832).IHC was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with LAT2 Mouse mAb (Ab112832) at 7.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm and membrane.
LAT2 Mouse mAb (Ab112832) - Western Blot All lanes: LAT2 Mouse mAb (Ab112832) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 58 kDa Observed band size: 27 kDa Exposure time: 100.0 s
ZJ24F0708420
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