ExactAb™,已验证,无载体 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Mouse anti Human NAGK Antibody, Monoclonal (K1E005_8C10), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/50-1/250
IP: 1/100
Protein Function
Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.
NAGK Mouse mAb (Ab117026) - Western Blot
All lanes: NAGK Mouse mAb (Ab117026) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 11.4 s
NAGK Mouse mAb (Ab117026) - Western Blot
All lanes: NAGK Mouse mAb (Ab117026) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 35 kDa
Exposure time: 80.6 s
NAGK Mouse mAb (Ab117026) - IHC
IHC analysis of tonsil tissue by NAGK Mouse mAb (Ab117026).IHC was performed using sections of the formalin-fixed paraffin-embedded tonsil tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NAGK Mouse mAb (Ab117026) at 2.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Germinal center cells and non-germinal center cells are positively stained at the cytoplasm.
NAGK Mouse mAb (Ab117026) - IP
Immunoprecipitation was performed by incubation of 2.5 µg NAGK Mouse mAb (Ab117026) with HeLa lysate containing 200 µg total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. NAGK Mouse mAb (Ab117026) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.Lane 1: HeLa lysateLane 2: NAGK immunoprecipitated from HeLa lysate by NAGK Mouse mAb (Ab117026)
Lane 3: The isotype control antibody instead of NAGK Mouse mAb (Ab117026) in HeLa lysate.Result: NAGK Mouse mAb (Ab117026) can immunoprecipitate NAGK.
1/1000
1/100
1/50-1/250
| 作用机制 | Action Type | target ID | Target Name | Target Type | Target Organism | Binding Site Name | 参考文献 |
|---|
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ24F0809840 |
分析证书 | Ab117026 |
| ZJ24F0809839 |
分析证书 | Ab117026 |
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